Chemical Composition And Biological Activity Of Viscum Coloratum And Viscum Liquidambaricolum

As a kind of medicinal plant, Viscum coloratum (Kom.) Nakai has been use d for hundreds of years. Mistletoe is mild-natured, bitter taste. Mistletoe plays an important role in making bones and muscles strong, tonifying the liver and kidn ey, curing rheumatism and pharmacodynamic. Pevious studies indicated that many compounds of mistletoe had antioxidant activity, antitumor activity, antiviral acti vity and antiarrhythmic activity. As the same genus of V. coloratum, the whole plant of Viscum liquidambaricolum Hayata. is widely used in curing rheumatoid arthritis, lumbar muscle strain. We persued investigation of the chemical constitu ents and biological activities of V. coloratum and V. liquidambaricolum. Some co mpounds were tested for their cytotoxic and antioxidant activities.By silica gel, ODS-AQ-HG, HW-40F, Sephadex LH-20and HPLC column c hromatography,14compounds were obtained from V.liquidambaricolum, including viscumneoside A (1), viscumneoside B (2), viscumneoside C (3), naringenin (4), eriodictyol (5), honoeriodictyol (6), honoeriodictyol-7-O-β-D-glucoside (7), eriodi ctyol-7-O-β-D-glucoside (8), naringenin-7-O-β-D-glucoside (9), pinocembrin-7-O-β-D-glucoside (10),4-hydroxyphenylacetic acid (11),4-[3’-(4"-hydroxyphenyl)-2’-acry late]-3-hydroxy-1-methoxy-7-oxo-6-oxybicyclo-[3.2.1]-octane (12),3-[3’-(4"-methoxy phenyl)-2’-acrylate]-2-oxo-tetrahydrofuran (13) and3-[3’-(phenyl)-2’-acrylate]-2-oxo-t etrahydrofuran (14). Compounds1,2,3were new compounds. The chemical stru ctures were determined on the basis of NMR, MS, UV and IR. Free radical sca venging activity results showed that compounds5,6,7and8showed strong DP PH radical scavenging activities. The whole plant of V.coloratum was investigated of its chemical compositio n.20compounds were obtained, including oleanolic acid (15), betulinic acid (16), amyrin (17), amyrin acetate (18),(20R,22E)-cholesta-5,22-diene-3a,4β,21-triol (19), pyran[3,4-b]indole-2-ketone (20),3’,4’-dimethoxylquercetin (21),7,3’,4’-trimethoxyl flavone (22),3,7,3’,4’-tetramethoxyquercetin (23), amyl4-hydroxybenzoate (24), is obutyl4-hydroxybenzoate (25),4-isopentyloxy benzaldehyde (26),2-hydroxypheny lacetic acid (27),4-hydroxycinnamic acid (28),1,7-di-(4-hydroxyphenyl)-4E,6E-hep tadiene-3-ketone (29),1,7-di-(4-hydroxyphenyl)-1E,4E-heptadiene-3-ketone (30),5-h ydroxy-1,7-di-(4-hydroxyphenyl)-1-heptene-3-ketone (31), Bloom alcohol (32),β-sit osterol (33) and β-carotene glucoside (34). Some compounds were tested for thei r cytotoxic activities, the compound30showed some strong cytotoxic activity ag ainst the A549cancer cell line.We carried out a study on chiral separation of enantiomer of eriodictyol, dr acocephins A and lobechinenoid by using chiral HPLC technique. In the study, t he four enantiomers of lobechinenoid were chirally prepared and their absolute c onfigurations were determined.