Clinical Study Of Slow Trasit Constiption Of TCM Syndrome Differentiation And The Relationships Of Vasoactive Intestinal Peptide Substance P And STC TCM Syndrome

Objective:(1) To preliminary discuss the distribution law of slow transit constipationTCM syndrome types.(2) To study the relationship between substance P&vasoactiveintestinal peptide and TCM syndrome types, exploring the inner relation between themin order to search evidence of slow transit constipation among TCM syndrome typesobjective research and diagnosis&treatment, to improve the accuracy of clinicaldiagnosis of TCM and the patients in therapeutic effect.Methods:(1) To collecte the general situation and the TCM four methods diagnosticinformation and so on in our hospital patients with constipation. Reference to Rome Ⅲdiagnostic criteria, combined with colon transmission test,to collect slow transitconstipation patients231cases. the table for four diagnostic information according tothe traditional Chinese medicine can spleen and stomach disease branch to make "TCMdiagnosis and treatment of chronic constipation consensus (2009, Shenzhen)"[1]ofsyndrome different-iation, divided into five types: intestinal excess heat syndrome in39cases, intestinal qi stagnation syndrome in57cases, lung and spleen deficiencysyndrome in51cases, spleen kidney yang deficiency syndrome in38cases, jin lessblood loss syndrome46cases.(2) By random method, from the5syndrome group wererandomly selected15cases, corresponding into observation group of5groups, andanother15cases without constipation symptoms of normal healthy people as controlgroup, a total of90cases. Blood samples were collected for all patients, centrifugation,separation of serum, in-80℃refrigerator, using enzyme linked immunosorbent assay(ELISA), finally unified detection of serum vasoactive intestinal peptide (VIP),substance P (SP) content, and record the number. Apply SPSS17.0medical statisticalsoftware for statistical analysis. Results:⑴231cases of study patients, the TCM Syndrome Distribution: intestinal qistagnation syndrome (57cases), lung and spleen deficiency syndrome (51cases), Jinless blood loss syndrome (46cases), intestinal excess heat syndrome (39cases), spleenkidney yang deficiency syndrome (38cases).⑵The results showed: gender wasstatistics significant in STC TCM syndrome type distribution (P<0.05), there wassignificant difference in the age of STC TCM syndrome type distribution (P<0.01).⑶The concentration of serum VIP in STC five syndrome types was higher than that ofthe normal control group. Statistical result：①There were statistics significant betweenthe five groups of syndrome type and the control group (P <0.05).②Comparingintestinal excess heat group wiht intestinal qi stagnation group had statistics significant(P <0.05).③Comparing intestinal excess heat group wiht lung and spleen deficiencygroup,spleen kidney yang deficiency group and jin less blood loss group both hadstatistics significant (P <0.05).④Comparing intestinal qi stagnation group to lung andspleen deficiency group,spleen kidney yang deficiency group and jin less blood lossgroup both was significantly different (P <0.01).⑤There were no significantlydifference between lung and spleen deficiency group and spleen kidney yang deficiencygroup and jin less blood loss group.⑷And compared with the control group, the contentof SP in serum STC decreased in intestinal excess heat group and intestinal Qi group,other three groups increased. Statistical result：①The five types of syndrome group andthe control group had statistics difference (P<0.05).②Comparence between theIntestinal excess heat group and intestinal qi stagnation group showed no significantdifference (P>0.05).③Comparing intestinal excess heat group wiht lung and spleendeficiency group,spleen kidney yang deficiency group and jin less blood loss groupboth had significantly difference (P<0.01).④Comparing intestinal qi stagnation groupto lung and spleen deficiency group,spleen kidney yang deficiency group and jin lessblood loss group both was significantly different (P <0.01).⑤There were nosignificantly difference between lung and spleen deficiency group and spleen kidneyyang deficiency group and jin less blood loss group(P>0.05). Conclusion:⑴Middle-aged and aged people have a fair chance of the STC, andespecially the middle-aged women and older men. The STC TCM syndrome typedistribution had a relationship with gender and age.⑵The STC early stage belong toexcess syndrome, often manifest as performance the intestine excess heat syndromeand intestinal qi stagnation syndrome, and the latter period belong to deficiencysyndrome,often manifest as performance spleen and kidney Yang deficiency syndromeand lung and spleen qi deficiency syndrome, Jin less blood loss syndrome.⑶The VIPare associated with the occurrence of STC, and may become one of the main objectiveindexes,to identify the excess and deficiency syndrome,and the intestine excess heatsyndrome and intestinal qi stagnation syndrome of the excess syndrome.⑷The SP mayhave relationship with slow transit constipation of TCM, and will likely be one majorobjective indexes,to identify the excess and deficiency syndrome of STC.