| Purpose To examine the effects of sinomenine on gastric emptying, small intestinal motility and serum motilin regulatory role in normal mice.Methods65mice of conventional Kunming mice were provided by the Experimental Animal Center of Lanzhou University were used in the experiment, and were randomly divided into groups as high-dose-sinomenine, middle-dose-sinomenine, low-dose-sinomenine, saline control, and anisodamine control group, After prohibited water drinking for24hours, all of mice were administrated with100mg/kg of sinomenine to high-dose-sinomenine group,50mg/kg of sinomenine to middle-dose-sinomenine group,10mg/kg of sinomenine to low-dose-sinomenine group,10mg/kg of saline to saline control group, and8mg/kg of anisodamine to anisodamine control group by subcutaneous injection. After30min with2%dextran blue-20000.4ml gavage, respectively. Twenty minutes later, blood samples were draw from the mice, blue color front lines at small intestine were confirmed, gastric liquids were taken, and the distances from pylorus to the color front lines were measured for the calculation of gastric remnant and small intestine propelling rates. The ELISA was used to measure the motilin concentration in mouse serum.Results There are no statistically significant difference in the gastric emptying of low-dose group (90.99±7.57%), middle-dose group (88.42±8.18%) of sinomenine and Anisodamine group (75.94±10.39%) compared with than saline control group (84.39±14.2). Low-dose group, middle-dose group of sinomenine could increase mice gastric emptying rates than high-dose group (76.93±11.33%), with a statistically significant difference (P<0.01). Anisodamine group could decrease mice gastric emptying rates than low-dose group and middle-dose group, with a (P<0.05); High-dose group of sinomenine (32.35±8.75%) could decrease mice intestinal propulsion rates than saline control group (47.28±7.12%), anisodamine group (46.27±7.81%), Low-dose group (42.47±12.48%) and middle-dose group (43.63±15.80%), with a statistically significant difference (P<0.05); Compared to saline control group (176.72±61.6%) Anisodamine group (112.85±69.56%) could reduced the concentration of motilin, with a statistically significant difference (P<0.05), middle-dose sinomenine group (250.85±77.25%) increased the concentration of motilin, with a statistically significant difference (P<0.05); With the mouse serum motilin concentration increased, the gastric emptying rate also went up; and when motilin concentration gradually decreased, the rate of gastric emptying went down too. Thus motilin concentration was positively correlated with gastric emptying rate.Conclusion The effect of sinomenine on the gastric emptying performed two-way adjustment. Sinomenine promoted the gastric emptying in low-dose and middle-dose, while inhibited the gastric emptying in high-dose, small sample size may lead to no statistically significant difference in sinomenine and saline control groups. High-dose sinomenine inhibited small intestinal propulsion. Sinomenine significantly promoted the secretion of motilin in middle dose, while anisodamine inhibited the secretion of motilin. Motilin concentration was positively correlated with gastric emptying rate. |
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