Studies On Standardized Extracts Of Fructus Schisandrae

Fructus Schisandrae, originated from the dried ripening fruits of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), which is woody climber, is usually called ’Bei Wu-wei-zi’ in mandarin. S. Chinensis was applied in oriental medicine for a long history and ’Shen Nong’s Herbal Classic’ ranked it into the high grade.Standardized Extracts of Traditional Chinese Medicine refers to an extracted and processed product of Chinese crude drug with relatively definite medical substance and stringent quality specification; it can be applied as a medicine or crude drug in pharmaceutics. At present Standardized Extracts take possession of a large proportion of domestic and overseas botanical medicine market.In this study, a relatively allround research of Standardized Extracts of Schisandra was carried out with commence of enrichment of chemical constituents, including investigation of total count assaying methodology, definition of preparation procedure, isolation and elucidation of chemical reference substance, assaying of different habitat and collecting date and HPLC fingerprint analysis.Main research content and results are as follows:1. In this paper, the chemical composition, pharmacological actions, quality evaluation and Standardized Extracts were briefly reviewed and compiled.2. Total count assaying methodology of three types of chemical constituents were screened and ascertained; these assaying results can mostly reflect the realities of content determination.3. Integrated preparation procedures, parameters and criteria of six Standardized Extracts were definite in order to guarantee the quality consistency; furthermore; the chemical constituents were actually enriched in diverse extent to meet different requirements.4. Chemical reference substances were investigated by means of silica gel column chromatography, preparation TLC, Sephadex column chromatography, HPLC preparation chromatography and recrystallization. The standard reference materials for HPLC assaying and fingerprint were depurated.5. The qualities of materials originated different habitat and collecting date and those four Standardized Extracts were evaluated, including content assaying of one compound.6. The method of HPLC fingerprint spectrum on the studies of Standardized Extracts of Schisandra is applied for the first time; quality control pattern of the extracts with fingerprint was established; full-scale quality evaluation on Standardized Extracts originated different habitat and collecting date was set up. The rationality and correctness authenticated by cluster analysis method.7. Moisture and ash content were determined; the content of heavy metals was also analyzed with the method of atomic absorption spectrophotometry and chromatometry.