Synthesis And Application Of Metal Iridium Complexes To Explore Things And Nanoparticles

Phosphorescent metal complexes with light physical properties, have manyadvantages compared with other organic phosphorescent materials, such as,emission wavelength changed with the environment changes, the significant stocksshift and long emission lifetime. Long emission lifetime facilitates the use oftime-resolved techniques to effectively avoid the interference of backgroundfluorescence. As the advantages they have, the iridium complexes have been appliedin many fields like, OLED (Organic Light-Emitting Diode), catalysts, chemosensor, andbioimaging in living cells. In this thesis we designed and synthesized a series ofphosphorescence iridium complexesand studied their applications in cell markers,nucleic acid labeling, MR imaging and photodynamic therapy. The main work isdivided three aspects:1. A d-f heteronuclear probe for dual-modality phosphorescence imaging andmagnetic resonance imagingTaking advangtage of the d-f heteronuclear bimetallic complexes (Ir-Gd) as adual-mode biological imaging probe, and evaluate its application in magneto-opticaldouble mode in biological imaging. First, evaluate the cell cytotoxicity of Ir-Gd,followed by an investigation of the fluorescence and MRI imaging at the cellular level.Then imaging of MRI effects on mice was carried out. Finally, tissue distribution andpathological study of Ir-Gd was performed.2. Metal iridium complex and the application in phosphorescence imaging andphotodynamic therapyIn this chapter two new iridium complexes containing triphenylamine structurewere synthesized and the photophysical properties of the iridium complexes werecarried out. In vitro photodynamic therapy effect were studied. The cell toxicityshowed that, complexes1and2with low cell toxicity. The confocal experimentshowed that, complexes1and2can be used as a phosphorescent probe in cellimaging. According to in vitro photodynamic experiment,complexes complexes1and2could decrease the absorbance of DPBF at the410nm, means that, iridium complex1and2could act as a photosensitizer in photodynamic therapy.