Experimental Study Of Inflammatory Cytokines And Tumor-associated Blood Clotting Disorder Thrombophilia

Objection:To detected inflammatory factors and blood coagulation indicators expression in patients with tumor and non-tumor deep vein thrombosis (NT-DVT) respectively.To study the relativity between inflammatory cytokines and tumor associated thrombophilia.Methods:68patients were divided into tumor group and NT-DVT group.Tumor group included20Leukemia patients (17Newly diagnosed and3relapsed),10lymphoma patients,21lung cancer patients (18Newly diagnosed and3relapsed).Samples were collected1week after treatment in leukemia patients,1month after treatment in lung cancer and lymphoma patients.NT-DVT group included17patients. The sample was collected2weeks after anticoagulant therapy.Control group contained20persons who were blood donors.Their samples were collected before blood donation. All samples were stored with3.8%sodium citrate anticoagulation, centrifugal separation of plasma which was used to blood clotting tests,including prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT), fibrinogen(FIB), D-dimer(D-D), antithrombin-Ⅲ(ATⅢ),von Willebrand factor(vWF) by STA-Evlution automatic blood coagulation analyzer in2hours.The others were collected and stored into-80℃fridge after centrifugation.The plasma interleukin-1β(IL-1β), IL-18,tumor necrosis factor-α(TNF-α) and tissue factor(TF),cancer procoagulant(CP) were tested by enzyme-linked immunosorbent assay (ELISA).Results1. The expression of plasma IL-1β、IL-18and TNF-α in tumor group were higher than control group,after treatment the level decreased (P<0.05), especially the expression of plasma IL-1β was higher in NT-DVT group and lung cancer patients than that in leukemia patients.(P<0.05).2.The expression of plasma IL-1β、IL-18and TNF-α in NT-DVT patients were higher than control group,and decreased after anticoagulant therapy(P<0.05).3.The level of TF was higher than control group in NT-DVT and tumor group (P<0.05). After treatment the expression was decreased(P<0.05). Before treatment it was positive correlation between TF and inflammatory cytokines (P<0.05) in NT-DVT patients,while there was no correlation in tumor group. Before treatment,the expression of CP was higher in tumor group than in control group(P<0.05).and decreased after treatment.Compare with control group,there was no difference of CP in NT-DVT group before and after treatment. Before treatment there was positive correlation between CP and TNF-a (P<0.05),but no correlation between CP and IL-1β or IL-18in tumor group.4.The expression of plasma D-D,vWF,ATIII in tumor group was higher than control group before treatment and decreased after treatment(P<0.05). The expression of plasma FIB was higher than control group before treatment,and decreased after treatment in NT-DVT group,but no decreased in tumor group;D-D and vWF in NT-DVT group were higher than control group before treatment(P<0.05),after anticoagulant therapy,the level decreased (P<0.05),but the level of ATIII increased in NT-DVT group (P<0.05).Conclusion1.The expression of inflammatory cytokines (IL-1β,IL-18and TNF-a) were high in tumor group and in lung caner was higher than which in leukemia.The inflammation factor was one of important reasons induced tumor associated thrombophilia.2. TF and CP were important markers of tumor associated thrombophilia, but the expression of CP in NT-DVT was not obvious.3. The expression of plasma D-D,vWF,ATIII and FIB can be uesed as a predictor of tumor associated thrombophilia coagulation.4. Inflammatory cytokines promote high expression of TF,could be the mechanism of thrombosis in NT-DVT.To treat the tumor and NT-DVT could decrease the expression of inflammatory factors and blance the coagulation disorders.