, Electrical Stimulation Mechanism "Zusanli" "Quchi" Points Renal Function And Blood Pressure In Spontaneously Hypertensive Rats

Purpose：In this study, by observing the effection of acupuncture at Zusanli（ST36） and Quchi（ST11） on spontaneously hypertensive rats about rats systolic blood pressure and the contention of Nitric Oxide （NO）, prostacyclin2（PGI2）, AngiotensinII（AngII） to evaluate vasomotor cytokine effection bloodpressure; at the same time, discuss the effection of acupuncture SHR aboutthe content of Serum creatinine（Scr）, Blood Urea Nitrogen（BUN） in serum, and24-hour urine albumin（mALB）, beta2-microglobulin（β₂-MG） in urine toevaluate the effection of acupuncture on renal function in SHR; Observedthe influence of routine HE staining and Western blotting on the morphology of kidney tissue and the content of kidney Collagenâ… and â…£ to evaluateacupuncture effects on SHR rats kidney morphology; And further studied fromthe perspective of reverse transcription-polymerase chain reaction（RT-PCR）expressionof renal kallikrein mRNA and transforming growth factor beta1mRNA（TGF-β₁mRNA） to discuss the effect of electro-acupuncture at Zusanli（ST36）and Quchi（LI11） on the blood pressure and Kidney function in spontaneouslyhypertensive rats.Material and method：Using the random number table method to24malespontaneously hypertensive rats at the age of24weeks8in each groups divided into3groups: SHR group （daily bundled and fixed20min, no acupuncture）; Perindopril group（peridopril suspension0.4mg/kg/d fill the stomach）;Acupuncture group（bundled rats and needle ST36and LI115-7mm then insertion into the electric acupuncture device, strength is degrees with slighttrembling needle handle, one time a day）;The same age age germline8malenormal blood pressure Kyoto rats to WKY group（not any intervention）. Usingintelligent non-invasive blood pressure measurement in the rats initial blood pressure and after treatment for4hours,2weeks,4weeks,6weeks rattail artery systolic blood pressure changes, each measurement time for the experimental intervention after4hours.Continuous treatment for6weeks.After the end of treatment,the collected24hours urine of rats,with ELISAmethod to measure urine trace albumin in the urine（mALB）,urine beta2-microglobulin（beta2-MG） content;Abdominal aortic blood, part of blood serum,centrifugal separation using fully automatic biochemical analyzer todetectthe contents of serum creatinine and urea nitrogen; Portions of the bloodwith anticoagulant, centrifugal plasma collection, with ELISA method todetect the plasma nitric oxide （NO） and the top ring element （PGI2）, angⅡ（AngⅡ） content; In kidney tissue50mg in pathological morphology observation and HE staining immunohistochemical method to detect the expression ofcollagen type â… and â…£; Another kidney tissues around50mg with RT-PCRmethod to detect renal kallikrein mRNA, TGF beta1mRNA expression.Data is the single factor analysis of variance with the SPSS16.0software,comparing two groups using the LSD method analysis,and results expressedas mean±standard deviation （x±s）,P<0.05was considered significant difference.Results：1.Acupuncture Zusanli and Quchi on SHR about systolic blood pressure has good antihypertensive effect, similar to the antihypertensive effect of perindopril treatment group, and acupuncture instant depressurization effect ofwestern medicine group.2.Acupuncture Zusanli and Quchi improved SHR plasma vascular endothelial active substances NO, PGI2levels,reduced the conten of vasoactive substancesAngII,adjusted Vascular Endothelium balance function.3.Acupuncture Zusanli and Quchi significantly lower the contention in SHRserum urea nitrogen（BUN） and creatinine（Scr） and the urinary beta2-micro-globulin（beta2-MG） and of albumin （mALB） in urine, kidneyfunction improvedsignificantly.4.Acupuncture Zusanli and Quchi improve the SHR kidney tissue pathological structure, reducing the expression of of collagen typeâ… and IV, reduce glomerulosclerosis and renal interstitial fibrosis.5.Acupuncture Zusanli and Quchi raise the level of SHR kallikrein mRNA expression, reduce the levels TGF-beta1mRNA expression, play a role of kidneyprotection.Conclusion：1.Acupuncture Zusanli and Quchi has antihypetensive effect in SHR.2.Acupuncture Zusanli and Quchi in SHR plays a protective role of hypertensiverenal impairment.3.Acupuncture Zusanli Quchi through the renin-angiotensin system （RAS） andkallikrein kinin systesm （KKS） regulate vascular endothelial diastolic factorof NO、PGI2、AngⅡ、kinin release equilibrium and reduce the TGF-beta1expressionof SHR for hypertension and renal protection.