Tumor Mechanism Paridis Saponins

Rhizoma Paridis are genus of Liliaceae, also known as Zaoxiu.The medicinal part is the dried roots of the plants and shape of lumps and cylindrical. Modern pharmacological studies have shown that Rhizoma Paridis have the function of hemostatic eliminate pain, anti-inflammatory, tumor control and regulation of the immune. Now, more than50compounds were isolated and identified from the genus, the main active ingredient of Rhizoma Paridis is Rhizoma Paridis total saponin, RPTS.A lot of researches about cancer have been reported, such as stomach cancer, breast cancer, esophageal cancer, but little about osteosarcoma. Now, the studies of anti-tumor mechanism about RPTS is still in the early, drawing some important research results mostly on a single, but Rhizoma Paridis associated with the process of a comprehensive anti-tumor mechanism. Therefore,the carrier is Kunming mice transplanted osteosarcoma S180cells in this experiment for studing the antitumor effect of RPTS. To describe the antitumor effect of RPTS via tumor inhibition and survival time of carrier; To observe the anti-tumor effect of carrier via using HE staining techniques in different dose group of RPTS; using immunohistochemistry technology, To analyse of the effects of anticancer mechanism via detecting the expression of related cancer-promoting genes, tumor suppressor genes and cancer factor expression on the re-building of total saponins in different dose group of RPTS.Results of experimental are shown below:1. The RPTS can significantly inhibit the S180mice tumor effect.Mice were fed in different doses of RPTS (0.075g/kg,0.15g/kg,0.3g/kg) and the inhibit tumor rates were38%,41%and42%,CTX group(0.02g/kg) was56.5%. In comparison with the control group (fed distilled water), there were significant difference in each treatment group (P<0.01); RPTS can significantly extend the survival time (life span) of mice transplanted S180, with a clear dose-response relationship and life span of three doses were34%,44%and55%, there were significant difference in each treatment group (P <0.01).2. Studies of histology showed that the CTX group and RPTS, the high-dose group can effectively reduce the number of transplanted tumor cells, and compared with the model control group, there were significant difference in each treatment group (P <0.01), and the tumor cell structure of each group transplanted appear the chromatin edge set, degradation and "bubble" phenomenon, and the with the RPTS dose increased, the more obvious structural changes.3. The technique of immunohistochemistry is used in mice transplanted S180tumor cancer cells. To determine and analyze the expression of P53, VEGF, MDM2and p65-four genes in different treatment conditions (CTX group, low-dose group,middle-dose group and high-dose group of RPTS).The results showed that:The positive expression rate of the tumor suppressor gene P53in five different processing case, least in the control group, the CTX group, RPTS low,middle and high dose group positive expression rate is gradually increasing, and each group compared with the model control group, there was no significant difference in the PRTS low-dose,and in the CTX group, the middle and high-dose group of RPTS with significant difference (P<0.01); The positive expression rates of cancer-promoting gene MDM2in five different processing conditions are that the control group with the maximum expression,CTX group with minimum expression and low,middle and high dose group of RPTS with the dose increased expression was significantly reduced, compared with the model group, CTX group (P<0.01), the high-dose group of RPTS had significant difference (P<0.05), in low and middle dose group,there was no significant difference; Positive expression rate of VEGF in five different processing case are that the control group with the maximum expression, the CTX group with the minimum expression and low,middle and high dose group of RPTS with the dose increased expression was significantly reduced, compared with the model group, the CTX group (P<0.01), the middle and high-dose group of RPTS,with significant difference (P<0.05, P<0.01), in low-dose group of RPTS, there was no significant difference; The Positive expression rate of nuclear transcription factor p65in five different processing case are that the control group with the maximum expression, the CTX group with the minimum expression, and low,middle and high dose group of RPTS with the dose increased expression was significantly reduced, compared with the model control group, in addition to the low dose group of RPTS, each group showed a significant difference (P <0.01).Conclusion:RPTS can significantly inhibite the tumor growth of mice transplated S180and can significantly prolong the survival time of mice xenograft;The high and middle-dose group can significantly reduced the number of transplanted tumor cells and inhibit apoptosis structure occurred; RPTS lowered VEGF, MDM2and p65and up-regulation of the expression of the P53gene to inhibit tumor cell value-added growth, and to promote apoptosis of tumor cells.