Impact On The Function And Structure Of HPO Axis In Rats With Exercise-induced Estrous Cycle Suppression By Two Kinds Of Carbohydrate Supplement

ObjectiveThe negative energy balance is a key factor caused exercise-induced menstrual disorder, The relationship of the energy balance and the reproductive system, as is the theoretical foundation of this study. This research tried to explore the possibility that carbohydrate supplement would delay the time of estrous cycle restrain and improve the status of estrous cycle restrain in rats under the same exercise intensity. In addition, the correlation between the energy metabolism index and sex hormones index is to be discussed.MethodDivided72two months old SD rats randomly into eight groups:the blank group(Con group, the negative control group), amenorrhoea model group(M group, positive control group),6D group(the oligosaccharides in which the energy were30%of normal diet added to rats in six weeks’ increasing load exercise),6G group(30%energy of normal diet added to rats by glucose in six weeks’ increasing load exercise),3D group(30%energy of normal diet added to rats by oligosaccharides, keeping on exercising till the end of9weeks),3G group(30%energy of normal diet added to rats by glucose, keeping on exercising till the end of9weeks),3U group(normal diet, keeping on exercising, same intensity) and3Q group (normal diet, resting after six weeks’increasing load exercise).6D group and6G group are to explore the possibility of energy supplement whether delay the time of estrous cycle restrain or not;3D group, the3G group,3U group and3Q group are to explore the different effects of different recovery methods to improve the status of estrous cycle restrain. Vaginal exfoliative cytological examination was been done to rats every night to figure out their estrous cycle conditions. With the reference to Bedford animal exercise load standards,3D group,3G group,3U group had done exercises for nine weeks,3Q group recovered quietly after six weeks; M group,6D group and6G had done exercises for nine weeks,3weeks behind other groups. One more exercise was added to the rats’ last training. Then all rats were put to death after a36hours break, in which the last eight hours without diet; firstly took6-8ml blood from inferior vena cava, after separating serum, the gonadotropin releasing hormones(GnRH), follicle-stimulating hormone (FSH), corpus luteum erythropoietin (LH), prolactin (PRL), progesterone (P), estradiol (E2)were tested.Results1. Weight of rats in M group is the minimum. The body weight rate decreased significantly to others.The weight, fat weight ratio of6G、6D、3G and3D groups decreased significantly.2. The recovery time of estrous cycle of3U group decreased significantly. 3. The ultrastructure of the HPO axis form in different level and M group show unusual changes4. The arcuate nucleus neuron myelin sheath detached in6G,6D prevent group. The arcuate nucleus neuron in3G,3D group recovered. The GnRH level of M group decreased significantly.5. The extent of damage for the Gn cells of6G,6D group is less than M group. The PRL level of3Q group and3D group is less than M group obviously.6. Rats’ovarian granular cells ultrastructure of6D,6G group damaged slightly. Meanwhile, the P level decreased significantly to M group. The mitochondria of3D,3G group increased obviously. Meanwhile, the E2level increased better than M group and3U group.The P level decreased significantly.7. The rats’endometrial epithelium cells ultrastructure of6D,6G damaged slightly. Microvilli of3G,3D group recovered faster and there were no differences between two groups. The recovery status better than3U group and3Q group.Conclusion1. Carbohydrate supplement in static state may cause the weight increasing significantly, but carbohydrate supplement in motor process may not.2. The internal sex hormone level be adjusted, which improve the reproductive function and delay the time of estrous cycle restrain in rats consequently. And it is showed that different kinds of carbohydrate supplement have no differences on effect.3. The carbohydrate supplement to rats at the time of estrous cycle restrain can adjust their energy condition in time, improve the central energy metabolism condition, and adjust the internal sex hormone level and cellular structure of HPO changed unusually at different level, thereby it can improve the status of rats’ estrous cycle restrain.4. The adjusting of the training programs can also improve the status of rats’estrous cycle restrain, although more time is needed.