Experimental Study On OPG And TGF-β1in Exhaustive Mouse Exposed To Natural Cryogenic Freezing Rain And Snow Environment

Objective: This thesis aims to explore OPG and TGF-β1in skeletalmuscle，skeletal and brain of mouse at quiet and exhaustive state exposed to naturalcryogenic freezing rain and snow environment, clarify variation and mechanism ofOPG and TGF-β1in mouse exposed to natural cryogenic freezing rain and snowenvironment, and then provide basic data for working training and physical forecast incryogenic freezing rain and snow environment. Methods: Eight weeks old maleKunming mice were subjects, after one-week feeding,randomly divided into controlgroup, exhaustion control group,7day exposure control group,7day exposureexhaustion group, with10mice in each group. Observe the mice general situationevery day, including weight, food, water, color and behavior. Control group wereexecuted when they have lived7days in normal temperature and humidityenvironment, and exhaustion control group were executed after exhaustive swimming.The mice belong to7day exposure group exposed to natural cryogenic freezing rainand snow environment1hour, continuously exposed7days, and they were feed innormal temperature and humidity environment usually.,7day exposure control groupwere executed and7day exposure exhaustion group were executed after exhaustiveswimming when they have exposed7days.we should drawn their skeletal muscle,skeletal and brain from them after they were executed. OPG and TGF-β1in skeletalmuscle，skeletal and brain would be measured by ELISA. Results:(1) Compared withcontrol group, OPG of skeletal muscle belong to exhaustion control group obviouslydecreased（P＜0.01）, and OPG of brain obviously decreased（P＜0.05）, but OPGin skeletal obvious increased（P＜0.01）.(2)Compared with control group, TGF-β1ofskeletal muscle of exhaustion control group distinctively increased（P＜0.01）, butTGFβ1in skeletal obviously decreased（P＜0.01）, TGF-β1in brain between controlgroup and exhaustion control group had not marked differences.(3) Compared withcontrol group, OPG in skeletal muscle and brain of7day exposure control group bothobviously decreased（P＜0.01）, but OPG in skeletal of7day exposure control groupdistinctively increased（P＜0.01）.(4) Compared with control group, TGF-β1inskeletal muscle and brain of7day exposure control group had not marked difference,but TGF-β1in skeletal of7day exposure control group obviously decreased(P＜0.01).（5）Compared with7day exposure control group, OPG in skeletal muscle of7day exposure exhaustion group distinctively decreased（P＜0.05）, OPG in skeletalobviously increased（P＜0.01）, but OPG in brain had not marked difference betweenthe two.(6) Compared with7day exposure control group, TGF-β1in skeletal muscleof7day exposure exhaustion group distinctively increased（P＜0.01）,but TGF-β1inskeletal obviously decreased（P＜0.01）, TGF-β1in brain distinctively decreased（P＜0.05）.(7)Compared with exhaustion control group, OPG in skeletal muscle andskeletal of7day exposure exhaustion group both obviously increased（P＜0.01）, butOPG in brain had not marked differences.(8) Compared with exhaustion control group,OPG in skeletal muscle of7day exposure exhaustion group obviously increased（P＜0.01）, TGF-β1in skeletal obviously decreased,TGF-β1in brain had not markeddifferences. Conclusions:(1)High levels of OPG in skeletal is the prerequisite thatphysiological function of motion system runs normally, skeletal muscle and brain arethe primary organs to supply OPG, as the stress conditions deteriorated increase, moreorgans may supply OPG.(2)High levels of TGF-β1in skeletal muscle is the effectiveprotection that physiological function of motion system exerts effectively in theprocess of exercise stress involving, skeletal is the main organ to supply TGF-β1，and brain involves in regulation as an auxiliary regulate organ.(3)OPG and TGF-β1complement and promote each other in maintaining the physiological function ofmotion system running normally.