Hazard Assessment Of Acrylamide Risk Based On HepG2 Cell Model

Since acrylamide was firstly detected in food in2002，the mechanism oftoxicity of acrylamide and mitigate strategy have been a hotspot in extensive research.The mechanism of formation and mitigation had been matured through a seires ofresearch. However, because of the limitation of research method, in vivo animal, itsrapid detection and real-time monitoirng methods are restricted. In addition, animalexpeirments to deduce human trials have yet to be veirifed. Therefore, it is necessaryto establish a human cells in vitro research system. Risk assessment of acrylamideincludes hazard identiifcation, hazard characterization, exposure assessment, irskcharacterization and irsk management. The ifrst three parts are relative to our research.This article used toxicology expeirments, analysis of systems biology and chemicalanalysis for the three parts. Establish the HepG2cell model and use2.5,5,10,20mMconcentrations and6h,12h,24h treating time of acrylamide treatment, which foundthat the shape of cell turned rounded when the concentration reached10mM，then usedouble staining method to get that cell proliferation would be restrained and apoptosisappeared above lOmM and12h; Obtain the reaction pirnciple of cells to the drug bytranscriptomics and metabolomics analysis and identify the biomarkers for the rapidmonitoring; UPLC-MS method of chemical analysis has been established foracrylamide detection in the cell matrix, not only the traditional intake monitoirng butalso the acrylamide controling truly act in human body.