Study On Phototoxicity Of Prussian Blue Nanoparticles

With the development of science and technology, interdiscipline has become a major trend, in which the application of nanomaterials in the medical field has drawn much attention. Because of their unique advantages in biomedical, nanoparticles are expected to solve biomedical problems of traditional medicine methods, leading a new revolution of modern medical diagnosis and therapeutic methods. Researching new features of traditional biomedical nanomaterials has become a new trend. However, the present study on nanomaterials is not deep enough, their bio-security in human body can’t be guaranteed and the preparation of materials is complicated, expensive and severely limites their further development. Therefore, nanotoxicology emerged, combing with traditional toxicology and gradually developed into the basis of nanotechnology, materials science, biology, medicine, toxicology, pharmacology, etc., which is crucial for the safety of nanotechnology and human society. At the same time, with the rising of photothermal therapy, which takes the advantage of surface plasmon resonance effect of light converting agents after laser irradiation to generate heat, cancer cells can be killed by high temperature and cancer be cured.Prussian blue is an ancient reagent with simple preparation, cheap price and excellent light converting properties. Most importantly, the long-term clinical use of it has verified its reliable biological safety in the human body and metabolic pathways. Because of these advantages, Prussian blue will have the potential to solve the problems that most nanomaterials have such as unguaranteed bio-safety and expensive price once it is used in medical territory. Therefore, it has great value for research and application, though it has been used in biomedical because of its excellent photothermal property and biocompatibility. The toxic mechanism which killing cancer cells through photothermal effect remains unknown. In this essay, the photothermal effect, photothermal stability, cytotoxicity and photothermal cytotoxicity of Prussian blue nanoparticles(PB NPs) were studied. We analyzed the manner of death of four different cells, He La, Chang liver, 4T1 and L929 at different PB concentrations and laser power densities, and further explored the factors that influence the survival of these cells.