Effects Of Tricholoma Matsutake Protein Hydrolyzate On Intestinal Microecology In High Fat Mice

With the improvement of life quality, high fat diet has become a risk to the human health, the intestinal inflammation caused by excessive fat accumulation leads to intestinal microenvironment disorder, oxidative damage to body and lipid metabolism disorder. In the research protein extracted from Tricholoma matsutake was hydrolyzed, combined the technology of microbial molecular ecology(ERIC-PCR fingerprint technology, Real-time PCR technology, Illumina Miseq PE250 / PE300 sequencing technology) and metabonomics technology(GC-MS fingerprint technolo gy) is studied, under the effect of long-term high fat diet in mice, the evaluation of Tricholoma matsutake of gavaging protein hydrolyzate the intestinal microecological changes affect how the body.This main research results are as following:(1) The protein of Tricholoma matsutake dry powder was 14.12%. the hydrolyzed protein degree of hydrolysis was 24.54%, contained 16 kinds of amino acids, 7 kinds of essential amino acids, the ratio of balance and rich degree is high, and rich in antioxidant activity amino acid;(2) The Tricholoma matsutake protein hydrolysate could improve high fat mice activity, slow weight gain and the accumulation of abdominal fat in mice, compared with the HFD group, HFD+T group serum CHO, TG, HDL, LDL and MDA level was reduced by 19.82%,19.82%, 19.27%, 15.00% and 19.27%, respectively, SOD, T- AOC and LPL activity increased by 11.51%, 14.04% and 42.85%,respectively, with significant difference(P﹤0.05), increase the body’s antioxidant capacity, weakened the dyslipidemia caused by high- fat diet;(3) ERIC-PCR fingerprint analysis of polymorphism,high- fat diet and Tricholoma matsutake protein hydrolysate has all stable effect on the intestinal flora, increase the diversity of the intestinal flora, high- fat diet influence of the intestinal flora is stronger than on Tricholoma matsutake protein hydrolysate. Real-time PCR analysis on key flora, Tricholoma matsutake protein hydrolysate increased under the high- fat diet the quantity of Bifidobacterium and Bacteroides, reduces the quantity of Escherichia coli and Enterococcus, normal diet, and increased the quantity of Lactobacillus and Clostridium leptum, showed Tricholoma matsutake protein hydrolysatecan regulate key flora;(4) High throughput sequencing test report indicated, it is concluded that 83222 sequence optimization,contained kinds of class level detected 9, kinds of genus level detected 66, kinds of classification level detected 133 kinds, including Alistipes etc. 8 kinds of bacteria in ND+T group and HFD group than ND group and HFD group has different degree increased significantly, 5 kinds of bacteria such as Bacteroides, except ND group,HFD group, HFD+T group, ND+T group amount increased. Tricholoma matsutake protein hydrolysate can change the flora structure, increase the number of the body to produce short chain fatty acid bacteria, reduce the accumulation of conditional pathogenic bacteria, increased the intestinal flora species abundance,diversity and species evenness;(5) GC-MS technology certain 118 kinds of substances.Among 7 kinds of short chains of amino acids substance, 16 kinds of long chain fatty acid, 19 kinds of sweets, 7 kinds of sugar acids substance, 7 kinds of benzene and 2 kinds of B vitamins, and many other complex metabolic products. Spectra analysis, Tricholoma matsutake protein hydrolysate obviously increases the body’s short chain fatty acids, amino acids and vitamins;(6) Tricholoma matsutake protein hydrolysate to change the quantity of Bifidobacterium、Blautia、Anaeroplasma、 Alistipes and S24-7_norank, thereby change the levels of SO D, T-AOC, D-Galactose, D-Glucose, Hexadecanoic acid and α-Linolenic acid, and improve the body’s antioxidant ability, promote the beneficial role of matter accumulation and accelerate lipid metabolism;To sum up, Tricholoma matsutake protein hydrolysate can regulate the intestinal micro ecology for high fat mice, can improve the intestinal flora diversity, abundance, good bacteria, reduce the accumulation of harmful bacteria, promote produce short-chain fatty acids, amino acids, vitamins, and other special bacteria, thereby increasing the body’s antioxidant capacity, weakening the body lipid metabolic abnormalities, achieve alleviate oxidative stress damage caused by a high- fat diet.