Based On The Three Dimensional Paper Gold Electrode Biological Photoelectric Functionalization Build Immunesensor

In parallel with remarkable progress in nanotechnology and bioconjugation techniques, new strategies have been developed using nanoparticles and nanostructured surfaces for electrochemical detection of cancer biomarker proteins. Low-cost, reliable, portable, multiplexed protein detection devices, especially those coupled with microfluidics, may soon enable accurate detection of panels of cancer biomarkers in blood, urine, sputum, or saliva. New signal amplification strategies will require proper attention to the nonspecific adsorption issues that usually control detection limits in electrochemical immunoassays. Significant progress in signal amplification has been made by using multienzyme labeling on metal nanoparticles, magnetic beads, and carbon nanotubes allowing ultrasensitive detection of protein biomarkers. A major challenge for electrochemical biomarker sensors is the reliable, simultaneous detection of multiple biomarkers in complex biological samples. The integration of EC and ECL immunoassays into a microfluidic format has created a strong platform for fabrication of devices for clinical diagnostics, particularly for POCT. The development and deployment of these systems could ultimately lead to more rapid clinical decision making and corresponding reductions in patient stress and healthcare costs.This article mainly adopting the wax printing and screen printing technology, using paper as base material, dedicated to the design and production of microfluidic chips. The integration of EC and ECL immunoassays into a microfluidic format has created a strong platform for fabrication of immunosensor for clinical diagnostics. Using nanoparticles and nanostructured surfaces could lead to an amplification of detection signal, allowing ultrasensitive detection of protein biomarkers. The researches carried out in this work described as follows:1 We developed a new type of high performance flexible 3D electrochemical paper based analytical device by the sequential growth of Au nanoparticles and electrodeposition of MnO2 nanowires networks on freestanding 3D origami device. We presented herein a simple immunosensing scheme and successfully fulfilled the highly sensitive detection of PSA.2 A sensitive microfluidic origami electrochemical immunodevice has been developed, based on polyaniline and Au nanoparticles modified paper working electrode as sensor platform. Using wax printing and screen printing technology, we design a kind of low cost, portable and disposable microfluidic chip. A highly sensitive multianalyte immunoassay was successfully developed for simultaneous detection of CEA and AFP.3 Using Au nanoflowers modified 3D paper gold electrodes as a sensor platform, we developed a novel ultrasensitive ECL immunoassay and successfully fulfilled the highly sensitive detection of AFP.