Synthesis And Characterization Of Dimeric Tridentate Chelating Ligands

Immobilized metal affinity chromatography as a novel isolation technology, it has become an important means of the isolation and purification of proteins. However, the leakage of metal ion from IMAC column is the fatal disadvantages of this method in its reality application, which makes serious pollution to the separation samples. As a result, the application of IMAC technology has been severely limited. Therefore, how to ensure the stability of IMAC column in its application has a very important actual significance. Clearly, developing new complexing ligand is of great significant to study the stability of IMAC column. It should be noted that the coupled to the substrate of chelator synthesis cannot be considered trivial.The dimeric tridentate chelating ligand has very strong binding force to metal ions. It can be put into use for the detection, fixation and purification of biological macromolecules. The dimerization of chelating agent can provide a cooperativity of binding of two adjacent immobilized metal ions to a histidine-tagged biomolecule, which has an edge on the bonding strength compared to a corresponding monomer chelating agent. In addition, a dimer enhances the selectivity against unmarked biomolecules with low metal affinity. The dimeric tridentate chelator in this paper has the following general formula as shown in figure(I), Sc is a scaffold or a connection structure, it contains two functional groups at the least, enabling coupling of two tridentate chelators(TP),and it’s structure as shown in figure (II).Paper in the second chapter designed the dimers chelating agent synthetic route.Third chapter with iminodiacetic acid and anhydrous ethanol as raw material, through acylation before the esterification method, they genarate of diethyl iminodiacetate, the optimum synthesis condition is:the mole ratio of alcohol acid is12:1, sulfoxide chloride dosage is0.125mol, reaction temperature is60℃, heat-up time is6h. Chapter iv to synthesis of diethyl iminodiacetate as starting material, choosing biological catalyst PLE for partial hydrolysis reaction, the iminodiacetic acid ethyl ester was genarated, its optimum synthesis condition is:0.1%of catalyst, reaction temperature is28℃, the reaction time is12h, the concentration of the buffer is0.184mol/L, pH value is7.5. Chose FT-IR, MS, NMR to characterize the above two kinds of ester.