Construction Of Composite Consortia FC1 And Its Characteristics Of Decomposing Corn Stalk

China produces more than 200 million tons of corn stalks as waste annually which can be turned into available resources achieved by means of stalks returned to the field.The growth of after-reap crop was restricted because of the slow decay process of returning stalks which limited the application of the technology quitely. Therefore, studies on the degradation microbial inoculun become more popular.The corn stalk degradation fungi were screened from sraw retuened soil and decaying woods by using corn stalk as the sole carbon source.Effects of surfactants on strain Z7-6 and F7-5 using corn stalk as substrate were studied by fermentation and degradation tests. Composite fungi consortia FC1 was constructed through complex methods and its corn stalk degradation effects were studied through potted simulation tests. The main results were shown as follows:Five corn stalk degradation fungi were screened from sraw retuened soil and decaying woods by using corn stalk as the sole carbon source.The five fungi strains were Penicillium oxalicum Z7-6, Aspergillus fumigatus F7-5, Trichoderma harzianum F4-3, Irpex lacteus L1-1 and Fusarium spp J2-5. The relative degradation rate of corn stalk was 52.66% by strain Z7-6 decomposing on corn stalk for 30 days. The five fungi strains could produce relatively high FPA,cellulase and xylanase,in which strain Z7-6 possessed the highest FPA (15.73 U/gds),CMCase activity (79.29 U/gds) and xylanase activity (1217.2 U/gds) in the liquid fermentation test, and its relative degradation rate of corn stalk was 37.75%.The fermentation and degradation tests proved that Triton X-100 in a 0.1% fluid concentrations could accelerate the process of strain Z7-6 decomposing on corn stalk obviously.Its relative degradation rate reached 61.7% through the liquid fermentation test for 7 days and 66.8% through the solid fermentation test for 30 days,which increased 24% and 14.2% respectively than the teratment without surfactantMeanwhile, all of 4 kinds of surfactants are able to promote the degradation rate of strain F7-5 decomposing on corn stalk.Best treatment of surfactants accelerate the process of strain F7-5 decomposing on corn stalk was add 0.1% Triton X-100 to the stalk surface. Its relative degradation rate reached 59.3% through the liquid fermentation test for 7 days and 60.7% through the solid fermentation test for 30 days, which increased 22.6% and 15.5% respectively than the teratment without surfactant.Composite fungi consortia FC1 was constructed through complex methods with four fungi strains included Z7-6,F7-5,L1-1 and J2-5.The con stalk relative degradation rate reached 49.32% through the composite consortia FC1’s liquid fermentation test for 7 days,it was almost 12% higher than strain Z7-6’s. The fungi strains complex experiments proved that increasing the degradation fungi species could promote the production of composite cellulose enzyme.At the same time, increasing the degradation fungi species would be helpful to enhance the stabilization of composite fungi consortia decomposing on corn stalk.The corn stalk degradation effects of composite consortia FC1 and Triton X-100 in a 0.1% fluid concentrations were studied through potted simulation tests. It was shown that FCl applied together with TR could effectively promote corn stalk degradation. Its degradation rate reached 57.1%, advanced 19.3% than the untreated corn stalk. The composite consortia FC1 had relatively high ability to decompose the cellulose, hemicellulose and lignin in the corn stalk.The degradation rate of cellulose, hemicellulose and lignin reached 50.8%,69.2% and 42.3%. It was shown that FC1 applied together with TR could effectively decompose the lignocellulose of corn stalk, its relative degradation rate reached 39.5%.