Study On Extracting And Purifying Sasanqua Saponin From Process Water Produced In Oil-Tea Camellia Oil Extraction

Using the aqueous phase byproduct referred to as process water of oil-tea camellia seed oil or process water, produced in extraction process of oil tea camellia seed oil by aqueous enzymatic method as raw material to study the technological conditions of sasanqua saponin extraction. At first, the crude products of sasanqua saponin was extracted by aqueous ethanol and by flotation machine. Secondly, the macroporous resin was used to purify crude sasanqua saponin to obtain the high purity product. Finally, the extraction technology of sasanqua saponin with ethanol method was optimized by orthogonal test, and the desorption process for purification of sasanqua saponin with macroporous adsorption resin was optimized by response surface method. The main results were described as follows:1. The main compositions of process water were determined. The results showed that the predominant chemical compositions were sasanqua saponin 5.98%, polysaccharose 6.14%, protein 5.12%, total flavonoids 0.047%. moisture 80.38%.2. The aqueous ethanol was used for the solvents in extraction to extract sasanqua saponin from the process water. The four factors including ethanol concentration, temperature of the extraction solvent, extracting time and pH value were investigated by single factor experimental and orthogonal test respectively to examine the impact on the extraction and purification of sasanqua saponin at different levels. In that case, the optimized process parameters were acquired. They were that the temperature of extraction solvent was 60℃, ethanol concentration was 40%, extracting time 120 min, pH value 4. Under the optimized condition the extraction rate of sasanqua saponin was 36.14% and the purity of sasanqua saponin was 50.21%.3. The foaming and stable foam properties of sasanqua saponin were utilized to explore if it could be extracted from process water by using flotation machine. Experiment proved that this method is really practical. The crude sasanqua saponin was extracted successfully. At the same time the research had carried out which investigate the factors such as impeller’s speed, flotation time, inflating volume, flotation time and flotation number inpact on the extraction rate of sasanqua saponin by single factor test. The test has acquired better process parameters. They were The impeller speed 2800r/min, flotation time 30min, aeration rate of 250 m3/h, the flotation number of 2, respectively. Under the optimized condition the extraction yield was 48.86% and the purity of sasanqua saponin was 53.56%.4. The DM130 macroporous resin was selected for sasanqua saponin purification by compared the adsorption and desorption capacity among with five different resins, single factor experimental and response surface analysis were adapt to study the process conditions of purifying sasanqua saponin with DM130 macroporous resin. The experimental results show that the DM130 macroporous resin was more suitable for the purification of sasanqua saponin as its faster adsorption rate and higher desorption rate. Therefore, it had higher adsorption rate than others when the concentration was 20.08mg/mL and the time was 120min. The optimal desorption condition was:the ethanol concentration 90.7% in desorption solution, desorption time 93 min and dosage 43.2mL/g. Under this condition the pureness of sasanqua saponin could reach up to 84.35%.