| Lonicera planting and processing bring a large number of abandoned leaves, result in environmental pollution and waste of resources. Extracting high added value product chorogenic acid from lonicera japonica leaf can achieve the reduction treatment and resource utilization of it. The extraction technology of enzymatic hydrolysis on chorogenic acid was concerned and enzymatic activity and appropriate conditions were studied,providing a new method for achieving reduction treatment and resource utilization.To obtain high hydrolytic enzymes, this paper through comparing different enzyme of catalytic degradation of lonicera japonica leaf, screened highly activity of cellulase, laccase and xylanase catalyzing plant fiber tissue. and transformation of pectinase on chlorogenic acid was found. The advantage ratio of compound enzyme was obtaind by using uniform design method and SPSS analysis method, To investigate the enzyme catalysis performance, SEM BET, and FTIR analysis were used to analyze the phase structure and the composition change of plant tissue.In order to promoting the extraction rate of chlorogenic acid, the suitable conditions for the extraction process of chlorogenic acid from lonicera japonica leaf by compound enzyme hydrolysiswas optimized by using response surface method The kinetic model of chlorogenic acid infiltration was established by using the particle diffusion equation. To achieve the high value transformation of chlorogenic acid, combined with industrial production and application,the transformation of pectinase on chlorogenic acid in diffirent pH conditons and pectinase dosage was discussed pectinase transformation mechanism on chlorogenic acid was also anaiyzed.By making up simulative sun cream to study the ultraviolet resistance of chlorogenic acid,provide experimental basis for product development of chlorogenic acid. The experimental conclusions were as follows:(1)The different enzyme of catalytic degradation and chlorogenic acid leaching from lonicera japonica leaf were compared, the highest leaching quantity was the compound enzyme(cellulase, laccase and xylanase). Used uniform design to explore the advantages of composite enzyme ratio, and throughed the SPSS to design the experimental results of the fitting analysis, it selected out the best catalyst added ratio was cellulase:laccase:xylanase=201:1883:35, the model’s R was 0.99, F-value was 103.09>9.3, It indicated that the experimental regression analysis was significant, the interaction of the selected factors had a significant effect on the experimental results. Interaction analysis of the selected factors showed that the interaction between cellulase and xylanase had a significant effect on the experimental results, Verified by the experiment, the deviation of chlorogenic acid leaching quantity was 1.3‰in the optimizing ratio, the experimental value was close to the theoretical value,and higher than any level value of uniform design,the scheme is feasible.(2)It selected and investigated the pH value, extraction time, hydrolysis temperature and enzyme dosage, used the Design-Expert 8.0 software to carry on the multiple regression fitting, the model’s P=0.0001<0.01, indicated that the model’s quadratic equation was highly significant, and the fitting degree of the model and experiment was fine. The optimum conditions were as follows:pH was 2.82, extraction time was 2.42h, hydrolysis temperature was 49.72,compound enzyme was 0.05g, predicted value of chlorogenic acid content was 5.28 mg/g, verification experiment result was 5.03 mg/g, the deviation was 3.8%. Using the particle diffusion equation, The kinetic model of chlorogenic acid infiltration was obtained, enzymatic hydrolysis dissolution of chlorogenic acid was concluded for three stages. Diffusion model fitting in a straight line, the correlation coefficient R> 0.96, it explained the kinetic of the dissolution of chlorogenic acid leaching in the enzymatic hydrolysis system effectivly.(3) the transformation of pectinase on chlorogenic acid in diffirent pH conditons and pectinase dosage was discussed, high content of chlorogenic acid and caffeic acid coulld be obtained in the condition of pH3 and With the increase of pectinase dosge, chlorogenic acid and caffeic acid linearly reduce or increase, chlorogenic acid could be completely transformed into caffeic acid at the point of 0.15g. Pectinase transformation mechanism on chlorogenic acid was studied, speculating pectinase could fracture ester base key, hydrolyzing chlorogenic acid into caffeic acid and quinic acid.(4) Coarse extraction fluid was used to the simulation preparation of sunscreen, the absorption of sunscreen on UVB ultraviolet were greater than 1.0,it showed that the paste keeping good ultraviolet resistant effect. |
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