Effects Of Dietary D-allulose On Plasma Glucose Management And Lipid Metabolism In Rats

Chronic diseases including diabetes and obesity have been popular because of unreasonable diets and eating habits. The traditional sweetener, sucrose has many disadvantages with lipogenic potency and high insulinogenicity, so it needs to be replaced. D-allulose is a new type of sweetener, which has 70% the sweetness of sucrose but only 0.3% energy of sucrose. Therefore,D-allulose is an ideal sucrose substitute for food products. It is meaningful to study on blood glucose and lipid metabolism of D-allulose in rats.This study was conducted to investigate the effect of D-allulose on the blood glucose. 40 male Wistar rats(4-weeks-old) were randomly divided into five groups: control group, glucose group, fructose group, D-allulose group and cellulose group. Oral glucose tolerance test and diurnal variation of plasma glucose were measured in each diet group of rats after seven weeks’ feeding of the experimental diets. Eight weeks later, half of the rats in each group were sacrificed at 6:00(preprandial) and the others were killed at 8:00(postprandial) to determine the serum glucose, insulin and the tissue glycogen. The results showed that the body weight in D-allulose group decreased by 13.4%, compared with control group. And plasma glucose levels were lower all day in the D-allulose group than other groups. At the same time, D-allulose could also improve the oral glucose tolerance. Besides, the data of sacrificing before and after meal showed that serum glucose concentrations both before and after a meal were lower in the D-allulose group than others, and insulin and liver glycogen were higher(p<0.05), but the solens glycogen and GSP were not relevant. Compared with control group, the D-allulose group could significantly reduce preprandial and postprandial blood glucose by 5.6% and 5.8%, respectively. Preprandial and postprandial hepatic glycogen increased by 28.2% and 39.3%, respectively.This study was conducted to investigate the effect of D-allulose on the lipid metabolism. 25 male Wistar rats(4-weeks-old) were randomly divided into five groups: control group, glucose group, fructose group, D-allulose group, and cellulose group. The rats were sacrificed after four weeks and the body weight, abdominal fat and organs weight, serum biochemical indexes, antioxidant capacity, and lipid metabolism related to enzyme activity were determined, liver and abdominal adipose tissue histological analysis were performed by microscopy. The mRNA expressions of ACCα, FAS, HSL, AMPKα2, PPARα, and SREBP-1c in liver were measured by RT-PCR. The results showed that compared with contrl group, the body weight, abdominal fat, and epididymal fat in D-allulose group decreased by 20.8%, 53.8%, and 49.3%, respectively; serum TG, FFA, LDL-C in D-allulose group decreased by 41.5%, 34.5%, and 51.1%, respectively; serum and liver’s SOD, and CAT in D-allulose group increased, improving the body’s antioxidant capacity; serum SDH and hepatic HL in D-allulose group increased, accelerating the metabolism of fat; histological analysis could protect liver cell damage induced and reduced hepatic fat deposition and adipocyte size. RT-PCR result showed that ACCα, FAS, and SREBP-1c were significantly decreased and expression of PPAR-α, HSL, and AMPKα2 were increased in D-allulose group.