| Porous starch was prepared from corn starch, and the effect of porous starch on blood lipids and cholesterol in ovariectomized rats was studied, then preparing the capsaicinoids porous starch microcapsules based on the slow release characteristics of porous starch and evaluating the it’s effect on the cholesterol and intestinal of the ovariectomized rats. The main reserch works were as follows:1. 24 SD rats were randomly grouped by weight(8/group), one group of pseudo surgery as the Sham control; the other two groups were ovariectomized bilaterally as OVX blank group and OVX porous starch group, feeding the corresponding feedstufs for 28 days.The study found that: compared with the Sham group, the OVX blank rats’ weight and abdominal fat mass increased, the serum TC, TG, LDL-C concentration, liver fat density, density and total amount of TG, TG were significantly increased, and the coprostanol, bile acid concentration and the total discharge amount in feces sinificantly reduced, at the same time, the free ammonia content and numbers of bacillus, lactobacillus, short chain fatty acid concentration in cecum contents decreased, the intestinal pH value increased. While, comparing to OVX blank group, the porous starch group rats’ weight and abdominal fat, serum TG concentration decreased significantly, HDL-C concentration increased significantly, AI and TG/HDL-C decreased significantly, liver fat concentration and total amount, TG, TC decreased significantly, fecal neutral sterol and bile acid concentration improved significantly. The substitution of porous starch could signicantly increase the number of bacillus and lactobacillus, increase the concentration of short chain fatty acids, decrease the pH value, there was no significant effect on the concentration of free ammonia. The study illustrates that 10% porous starch had a good lowering blood lipid function on hyperlipidemia rats induced by ovariotomy as well as high fat and high cholesterol diet, porous starch has a good regulation at disorder of lipid metabolism and intestinal environment changes caused by ovariotomy.2. Taking laboratory self-made porous starch as the carrier, prepared the capsaicin microcapsule microcapsules using the adsorption and slow resease characteristics of porous starch.Results showed that: the water absorption rate and oil absorption rate of self-made porous starch were increased about 40% compared with the original starch, the absorption performance was good. Centrifugaling and drying the the porous after adsorbed 1.5 h at 25℃ with 1 mg/m L capsaicinoids to make the capsaicinoids porous starch complexes, then taking gelatin as wall material to prepare capsaicinoids porous starch microcapsule. Wall material and complexes ratio was 1:1, stiring for spray drying at 40℃, air inlet temperature was 180℃, the material flow rate was 550 m L/h, the encapsulation efficieny was 86%, loading capsaicinoids 1.1mg/g and displayed certain slow release function of capsaicinoids at the protection of gelatin and porous starch in vitro experiments.3. 32 SD rats were randomly grouped by weight(8/group), one group of pseudo surgery as the Sham control; the other three groups were ovariectomized bilaterally as OVX group, feeding the corresponding feed for 28 days.The study found that: microcapsule and capsaicinoids both significantly reduced the body weight increments, abdominal fat, the TC, TG, LDL-C concentration in serum as well as the fat and cholesterol in liver significantly induced by ovariectomy, improved the anti arterial stiffness index, promoted the excretion of fecal neutral sterols and total bile acids. There was no significant difference between microcapsule and capsaicinoids in reduction of body fat, triglyceride in liver and serum, but the effect of reducing hepatic cholesterol and promoting cholesterol and bile acid excretion of microcapsule was better than that of capsaicinoids. On the other hand, capsaicinoids had no significant affect on bacillus, lactobacillus, and short chain fatty acid concentration in cecum contents, but the capsaicinoids damaged the small intestine and colon cells and villous, even caused the formation of intestinal tumors. While the capsule could significantly increase the number of bifidobacteria and lactobacillus in intestinal and improve the concentration of short chain fatty acid in the cecal contents compared to OVX blank group. Comparing to the Sham group, the rats that ate capsaicinoids porous microcapsule whose intestinal villis were sparser; there was damaged phenomenon in the colon and the bundle of the colon. There was an injury in the intestinal tract, but slightly less damaged than the capsaicinoids group rats. The study indicatied that the microcapsule which blocked the strong excitant taste of capsaicinoids had a good function of lowering blood fat and cholesterol, at the same time, relieving the harm and stimulation of capsaicinoids on the intestinal. |
PDF Full Text Request