Preparation And Properties Of Curcumin-chitosan Carrier

Curcumin,apolyphenolic compound extracted from the rhizome of the ginger family plant, is anorange yellow crystalline powder.Which is slightly bitter and insoluble in water but soluble in organic solvent, with a variety of pharmacologically active, such as anticancer, antioxidant, anti-inflammatory, lowering blood pressure, lowering blood sugar generation. But its bioavailability is very poor, mainly due to the rapid degradation of curcumin and elimination in the body, resulting in a lower plasma concentration. Therefore, it is very important to improve solubility, stability and bioavailability of curcumin. In this paper, the adsorption of chitosan was used to prepare curcumin- chitosan micros pheres with tripolyphosphateand glutaraldehydeused as crosslinking agent. The vehicle micros pheres were prepared with ion dipping aggregation, ion dipping aggregation, and emulsion crosslinking technique, respectively. The relationship between curcumin loading and process parameters were determined.Meanwhile the curcumin releasing in vitro simulation environment and stability of microparticle were determined respectively.The results as follows:1.UV spectrophotometry and high performance liquid chromatography can be used to measure the content of curcumin. HPLC method has high sensitivity, but the operation is complicated and high cost; UV points spectrophotometric method is convenient and quick, low cost, high recovery rate, the accuracy of the results meet the provisions of the Pharmacopoeia of the people’s Republic of China and suitable for determination of batch samples.2. The curcumin raw material was sensitive to changes of light, temperature, humidity, p H and other environmental conditions. Curcumin should be stored in environment without light, dry and low temperature.3. Compared with the preparation method of chitosan carrier, it was found that the ion implantation method was simple, and the drug release rate of the microspheres was moderate, which was suitable for the experimental study.4. In view of the release rate determination of curcumin chitosan microspheres in solution, the reverse dialysis diffusion is better than the positive phase dialysis diffusion, and the direct dialysis is better than that of the reverse dialysis method.5. Different methods were used to determinethe release of curcumin in vitro simulative gastrointestinal fluid, the reverse dialysis diffusion was found to be the most appropriate way tomeasure curcumin- chitosan solution in different media.6. The results showed that the stability of the curcumin was much higher than that of the curcumin powder after the encapsulationin chitosan microspheres. The light and temperature stability of the curcumin chitosan microspheres was significantly improved, and the humidity stability was slightly improved. The results showed that theencapsulationin chitosan microspheres could greatly improve the stability ofcurcumin.In summary,the stability and bioavailability of curcumin can be improvedby encapsulate in chitosan microspheres, so as to active drug bioactivity completely. Use chitosan microspheres as drug delivery system has a prosperous application and the research value.