Study On Separation And Purification Of Rapeseed Lecithin

Natural phospholipids,main constituents of biological membranes,are the physical basis of all vital movements.And phosphatidylchline,main active ingredient,have a lot of particular biological activities.But,our country spends much on importing expensive high-purity PC from foreign ountries.Therefore,it is necessary to study the process of separation and purification of lecithin.The project focus on the three parts as following.Firstly,the methods of definite quantity analysis and qualitative analysis were established from a large number data and basic theories.By using the method of high performance liquid chromatograpgy（the detector is evaporate light scattering detector）,the retention time of PC peak was 8.5min.Besides,the external reference method was used for qualitative analysis and the coefficient of variation of the retention time of PC were 0.13%and 1.16%,respectively.So the repeatability complies with the standard.Secondly,The technological process on the separation and purification of colza phosphatidylcholine（PC）from colza oil degummed residues was studied.PC was first extracted with 95%ethanol,aether and acetone,and then crude PC was used as raw materials to prepare high purity PC by column chaomatography of silica gel.The process was optimized by one factor experiment,Plackett-Burman experiment and Response-surface design experiment.The results showed that the best column chaomatography conditions for the process were:100 to 200 mesh silica as immobile phase;isopropanol-water（1∶1,V/V）as eluent;the loading amount was 0.084 g·（g sillca）^-1;the flow rate was 1.5ml/min;the maximum permissible concentration was 0.15g/mL;the ratio of height to diameter is 6:1.After being analysised by high performance liquid chromatography-evaporate light scattering detector（HPLC-ELSD）,the purity percentage and the recovery percentage of the product were 85.6 and 83.8 respectively.At last,the process of purification of PC were studied.Compared with other kinds of resins,D113 was the best immobile phase of the process,then,according to the purity and recovery of PC,the process was optimized,and the best column chaomatography conditions for the process were:the loading amount was 0.08 g·（g sillca）^-1;the flow rate was 3.0ml/min;the maximum permissible concentration was 0.1g/mL; the ratio of height to diameter is 10:1;isopropanol-water（2∶3,V/V）as eluent,the purity percentage and the recovery percentage of the product were 72.3 and 72.6 respectively.