Production Method And Clinical Application Of Compound Povidone Iodine Gel For Mastitis Treatment In Dairy Cows

Bovine mastitis cause the decrease of milk yield and quality which will bring serious economic losses in breeding industry of dairy cows. Nowadays, conventional antimicrobial therapy has been using to treat the bovine mastitis. Using a great deal penicillin, streptomycin and quinolone drugs ea al. causes residue pf antibiotics in fresh milk, which harm consumer’s physical and psychological health. Meanwhile, the using of antimicrobial will cause the pathogenic bacteria of bovine mastitis has drug resistance. Recent years, some new types of dispensarization have been using to treat bovine mastitis, such as the herbal therapy, biotherapy and iodine preparations therapy. The effect of these dispensarizations is not well. Productive technologies of some dispensarizations are not perfect. The treatment costs of some dispensarizations are rather high. These dispensarizations need further deep research. Therefore, finding a effective methods to treat bovine mastitis is scientific and technical workers’responsibility. This study is utilizing the antibiotic action of Povidone Iodine(PVP-I) and Hydrocortisone, mixing with other aided medicines and adopting orthogonal test to ensure medicine composition to make the Compound Povidone Iodine Gel (CPIG) using the breast perfusion for route of administration and appling the clinical pharmacodynamics test at the same time.In the study of the CPIG preformulation and quality controlling, We adopt orthogonal test to screen the optimum formula of it:sodium hydroxide is 0.075 g,4% PVP-I 3.75 g, potassium iodate is 0.50 g, and propylene glycol is 10 g in every 100 g the CPIG. The logarithmic linear relationship of heating time in various temperatures and available iodine’s concentration was good. The storage period was 799 d at 25℃. Hydrocortisone in the CPIG was determined by High Performance Liquid Chromatography(HPLC). The results showed that there was a good linear relationship within the hydrocortisone concentration range of 0.25～1.22 mg/mL(r=0.999 3), and the recovery rate was 96.15% with Relative Standard Deviation (RSD) of 0.41%. This experiment results indicate that the CPIG is worth further research and development in breeding industry of dairy cows. Meanwhile, the results provide theoretical basis for the CPIG production, storage and clinical practice.Its effect for treating clinical bovine mastitis is sound. The cure rate and effective rate are all higher than those of the other groups, the cure rate (79.41%) is very higher than RUYANDI control group (59.02%) (p<0.01), and the effective rate (92.65%) is higher than RUYOUXIAOYANJI control group (67.24%) (P<0.05). After treating subclinical bovine mastitis, its (71.02%) and (86.96%) are higher than MIRUTONG control group (64.52%,80.65%) and RUYANDI control group (63.08%,78.46%), the difference was significant (P<0.05).The results mentioned above indicate that the CPIG is of stable quality and safe for breast perfusion. All the clinical trials verify that the CPIG can be used for treating subclinical bovine mastitis and clinical bovine mastitis, efficiently, but producing no resistant strain or drug residue. It is convenient in operation and easy for spreading the application, which provide experimental basis for further study, manufacture and clinical application of new pattern of Gel (CPIG).