Study On Actinidia Kolomikta In Vitro Rapid Propagation

This study takes Actinidia kolomikta of Changbai Mountain as test materials and has atissue culture for the leaves of wild and plantlet leaves, petioles and stems by regenerationculture, to study its in vitro micropropagation system. this paper make a system analysis tosterilization method on leaves, explant induction and differentiation of callus, stem with axillarybuds induction, subculture, subculture cycle and times of callus and prolifera culture, rootingand transplanting to find out the best plant growth regulator combinations and culture conditionsin each stage of cultivation. The results are as follows:1. The best sterilization method of Actinidia kolomikta:0.1%HgCl2to sterilizing4min. Thecontamination rate was25%and the survival rate was45%.2. The best induction medium of leaf callus was MS+ZT1.0mg/L+NAA0.2mg/L, the callusinduction rate was91.67%. The best medium of callus differentiation of Actinidia kolomikta wasMS+ZT2mg/L+IBA0.2mg/L and differentiation rate was89.45%, budding is8.11.3. The best callus induction medium of leaf,petioles and stems of regenerated plants wasMS+ZT1.0mg/L+IBA0.2mg/L.stem segment was the best explant for callus induction ofActinidia kolomikta. Callus induction rate was98.07%.4. Valueof leaf tailed callus induction rate and differentiation rate was the highest, followed byleaf tip,and whole leaf was the lowes. Leaf tailed callus induction rate of0.5-1.0cm,1.0-1.5cmsize leaf was the highest,100%, the size of1.5-2.0cm blade tail differentiation rate was thehighest and the value was90.94%.5. The best induction and proliferation medium for stem section with axillary bud wasMS+6-BA2.0mg/L+NAA0.1mg/L.the germination rate reached100%and the germinationnumber is3.75. Effect of6-BA on the germination of axillary buds is better than ZT.6. The best optimum proliferation culture medium of Actinidia kolomikta was MS+6-BA3.0mg/L+IBA0.2mg/L+sucrose30g/L, the proliferation coefficient can reach more than5.82.7. Callus subculture in40d subculture cycle cultured to the fourth generation is preferred.Subculture proliferation with40d subculture cycle training to the fifth generation was the best effect, less variation in seedlings.8. The best rooting medium of bottle is1/2MS+IBA0.6mg/L.The rooting rate reached100%.The average root number was7.96and the average root length was3.70.With0.5mg/mLIBAsoaked,rooting rate and survival of plantlets is the highest, respectively64.98%and59.94%.9. The transplanting survival rate of plantlets was the highest in the matrix V (garden soil): V(peat): V (sand)=1:1:1and the value was92%; semi-shade treatment was in favor oftransplanting seedling survival.