The Study Of The Vitro Culture And Regeneration Plant Of Litchi And Longan

Litchi and longan are difficult in vitro culture, and the rate of the normal somatic embryo induction and regeneration plant is low, which have restricted the process of new variety breeding of them and development of the biotechnology. The shixia longan and Hinensis litchi in guangxi were taken as test materials, mainly studied the process of the anther, stem, blade and seed as the explants in vitro culture inducing the occurrence of callus, which had basically established the regeneration system of lizhi; And preliminary explored the process of somatic embryogenesis and plant regeneration by the isolated culture of longan seeds. Moreover, the stem segments of them were taken as the explants, which had respectively gotten the regeneration plant by the way of the organogenesis, this result had provided theoretical basis and technical support for the research of the basic theory of the biological technology and the genetic transformation and the new variety breeding of them. The results were showed as follows:1. The process of the anther, leaf, stem and seed as the explants in vitro culture inducing the occurrence of callus had gotten the different types of callus, the highest induction rate were82.1%,86.0%,81.3%and84.0%respectively. The media of better suitable for formation of embryonic callus of the litchi anther was:MS+0.1g/LVC+2.5mg/L2,4-D+0.5mg/L6-BA+1.0mg/L NAA+2.0mg/L KT+3%sucrose+0.7%agar.2. The culture medium of the subculture of the embryonic callus induced by the anther was:MS+1.5mg/L2,4-D+0.5mg/L6-BA+3%sucrose+0.7%agar and MS+l.Omg/L2,4-D+1.0mg/L KT+5.0mg/L AgNO3+3%sucrose+0.7%agar, by the way of the alternately successive transfer culture to get the stable lines of the embryonic callus, and successive transfer cultivated once21d, successive transfer cultivated4-5times. 3.The high-frequency culture medium of the somatic embryo was:MS+100mg/L inositol+0.1mg/L NAA+3.0mg/L CPPU+80ml coconut milk/L+5%sucrose+0.9%agar,with successive transfer cultivated2times under the weak light,the body embryo can be ripe.4.The better culture medium of the plant regeneration was:MS+100mg/L inositol+1.0mg/L IBA+0.1mg/L6-BA+60ml coconut milk/L+3%sucrose+0.7%agar,and there only had the somatic embryo with root,the rooting rate of somatic embryo is12.5%,and to get the complete plant,seedling and transplant need a further study.5.The stem segments of the axillary bud of the litchi and longan were taken as the explants to induce the stem segments budded directly,there had better gennination medium were:MS+0.6mg/L6-BA+0.2mg/L IAA+3%sucrose+0.7%agar、MS+0.6mg/L6-BA+0.4mg/L IAA+3%sucrose+0.7%agar,the germination rate was71.7%、75.6%,And the better suitable culture medium of induced bud seedling root was:MS+0.05mg/L6-BA+1.0mg/L IBA+3%sucrose+0.7%agar.MS+0.1mg/L6-BA+1.0mg/L IBA+3%sucrose+0.7%agar,the rooting rate was2.9%.5.0%.6.The seeds of Shixia longan were taken as the explants to preliminary induce regrowth by the way of somatic embryogenesis with in vitro culture,but no root,the induction of roots needs a further study.