Repair Replant Diease Of Cut Chrysanthemum Using By Phanerochaete Chrysosporium Chlamydospore

Replant disease is a common worldwide problem in limiting cut chrysanthemum production. Replantdisease is increasing with planting time, allelochemicals and pathogens in the soil year after yearaccumulation, and the cut chrysanthemum wilt is more serious with time. The remediation ofPhanerochaete chrysosporium against replant disease of cut chrysanthemum was tested in this paper. Themain results were listed as following:The reason and mechanism of cut chrysanthemum replant disease were stuied by testing four replantfields in Xinxiang which was continuously cropped for1,3,5and9years respectively. The results show thatcut chrysanthemum stem length gradully reduced and wilt diseases increased with increasing replantdisease years. The stem length of cut chrysanthemum planted5years was only66.67%of the one justplanted1year,and wilt disease was their12.00multiply. The content of urease in the cut chrysanthemumrhizosphere soil decreased with replanting time increased，while Catalase and Sucrase had no significantchanges in replanting. The CFU of fungi obtained from5and9replanting years soils are5.00and14.60tofirst time of their at1-year plant soil. The CFU of bacteria/fungi at first plant year are14.13and209.99to5and9replanting years. It revealed that phenolic acids especially p-hydroxybenzoic acid accumulation withcut chrysanthemum replant. The cropping obstacle of cut chrysanthemum may be related to phenolic acidsand microflora changes.Water culture experiment and pot culture experiment are designed to research P.chrysosporiumchlamydospore’s effect with chrysanthemum growth in replant factors. The results show that the leachliquor of cut chrysanthemum rhizosphere soil has a negative effect on seed sprouting and seedling growth,and this effect was increasing with leach liquor concentration.The addition of P.chrysosporium with leachliquor can reduce the hazard of leach liquor allelopathy for seed sprouting and seedling growth. Theaddition of P.chrysosporium in cut chrysanthemum rhizosphere soil has the ability to improve soilenzyme activity and CFU of bacteria. At the end of experiment, the CFU of fungi in T2（added2‰P.chrysosporium chlamydospore）and T5（added5‰P.chrysosporium chlamydospore）were48.71%and74.80%of the CK. The CFU of fungi in T2and T5were1.11and1.68time of the CK.P.chrysosporium chlamydospore was added to the soil which planted cut chrysanthemum for5yearsto research whether it may affect the quality of cut chrysanthemum in the replant disease land. The resultsshow that addition of P.chrysosporium chlamydospore can promote the growth of cut chrysanthemum.（1）The relative growth rate of T20（added20g P.chrysosporium chlamydospore） treatment and T50（added50g P.chrysosporium chlamydospore）treatment were more sensitive that the CK. The relative growth rateof T20treatment and T50treatment were increased37.74%and44.42%of the CK control at the first60days.T20treatment was1.20higher than the control at the end time of treatment.（2）Addition ofP.chrysosporium chlamydospore can slow phenolic acids especially p-hydroxybenzoic acid accumulationduring the treatment. The p-hydroxybenzoic acid of T20treatment and T50treatment was only57.40%and89.94%of the control at the end time of treatment.（3）P.chrysosporium can reduce the wilt disease of cut chrysanthemum. The wilt disease incidence of T20treatment and T50treatment were only19.77%and56.18%of the control at the end time of treatment.（4）P.chrysosporium can improve soil properties andmicroorganisms of cut chrysanthemum root. At the end time of treatment, The CFUs fungi of T20treatmentand T50treatment was49.06%and93.77%of the control. The CFUs of bacteria of T20treatment and T50treatment were1.47and1.65times of control. B/Fs of T20treatment and T50treatment were3.00and1.76times of the control.