Spores Of Phanerochaete Chrysosporium In High Yield Production Technology And The Application Of Soil Repair

The white-rot fungi biological technology is the typical product of the biological theory and application.The research is mainly focused on the study of the production and use of the chlamydospores ofPhanerochaete chrysosporium, a typical model biological of white-rot fungi. By controlling the cultureconditions of P. chrysosporium. Mycelium pellets which have certain mechanical strength and diameter can beproduced. We can cultivate the mycelium pellets to produce a large quantities of chlamydospores. Nowadays,other relevant researches concentrate mainly on the chlamydospores of Trichoderma. However, repots are rarethat chlamydospores are produced in quantity through liquid culture. Once formed into the chlamydospores,the fungi have a longer shelf life, so that they can be commercialized as a business. Three main parts areincluded in this paper.The first part a liquid culture condition where chlamydospore are produced by cultimating P.chrysosporium is introduced and optimized. Adding inducer-a of certain concentration into the culture mediumsuitable for P. chrysosporium's growing can promote the large production of chlamydospores. Optimizing theculture conditions, such as temperature, revolving speed, pH value, the major contents of the culture medium,will guarantee the best conditions to improving the production of chlamydospore. On the culture condition ofthe conidiospore: inoculation amount for1.1x10⁷a/mL, temperature33℃, speed180r/min, glucose20.0g,ammonium tartrate4.0g, MgSO₄·7H₂O1.5g, inducers-a3.5g, Tw800.5mL, benzyl alcohol0.5mL,KH₂PO₄2g, vitamin B₁0.001g, trace elements liquid70mL, pH4.5or so under the culture condition, the dryweight of mycelium pellets are4.1g/L, and the maxim output of the chlamydospore is25.4x10⁸a/L.The second part is to study the property of the chlamydospore is studied. The chlamydospore has apromising future of application in biological degradation, environmental protection and biological preventionand control. In view of this, this research will conduct the comparison study of the different effects that haveon the conidiophore and chlamydospore, such as germination temperature, culture medium, pH value,anti-ultraviolet characteristics, different processing methods and storage conditions. Different temperatureshave remarkable differences in the effects on the germination of the two spores. The germination temperatureof the chlamydospore ranges from10℃to45℃, and the suitable germination temperature's range is25-37℃;the germination temperature of the conidiophore ranges from25℃to37℃, and the suitable germinationtemperature's range is30-37℃, in the same temperature, chlamydospore germination is faster thanconidiophore, with its bud growing faster. Chlamydospores and conidiophores have a relatively less strictrequirement about pH value, The range of the suitable pH value for chlamydospore is1.5-9.0, with pH value of4.5the best; the range of the suitable pH value for conidiophores is1.5-9.0, with pH value4.5the best, whichindicates that it's proper for the spores to germinate in an environment which is neutral partial acid. Thegermination rate of the chlamydospore is about20%with ultraviolet (15W,20cm) irradiating90seconds,which totally restrains the germination of the conidiophores. Different also exist in the condition and time ofthe preservation of the two spores. When checked after being stored for120days in room temperature andnatural drying, the number of the chlamydospores falls from10⁸per gram to10⁶per gram while that of theconidiophores falls from10⁸per gram to10³per gram, which shows that the stress resistance of thechlamydospores is stronger than that of the conidiophores. Thus means the chlamydospores has an advantage over conidiophores in storage shelf life. Compared with the hyphae and the conidiospores, the chlamydosporehas a better potential of application and promotion to produce biological.The third part about the degradation of the chlamydospore to the herbicide atrazine is studied. As a resultof the wide use of the herbicide and its remaining in the soil, the ecosystem and human being's health will bedone harm to. We use the chlamydospore's stress resistance and special enzyme system to study thedegradation effect that the chlamydospore has on the long-lasting herbicide atrazine in the soil. Onthelaboratory condition,added to the soil, the Phanerochaete chrysosporium chlamydospore can colonies andgrow in it, degradations the atrazine in the soil through metabolism. In the simulated outdoor field, addingchlamydospores to the soil has a degradation rate of80-90%, reaching the goal of restoring the polluted soil.This research has a preliminary exploration in the application of Phanerochaete chrysosporiumchlamydospores microbial agents in the soil restoration.