Study Of The Rice Straw Biodegradation In Mixed Culture Of Phanerochaete Chrysosporium And Trichoderma Viride

China owned the highest yield of rice straw in the world. As a simple, cost-effective and sustainable method to dispose the rice straw, the microbial degradation of rice straw has received widely attention and study. The Phanerochaete chrysosporium, as an effective lignocellulolytic microorganism, possessed a broad application prospect. Trichoderma viride owned a high production of cellobiohydrolase enzyme, but its role in the degradation of lignocellulose and the degradation mechanism still received little research in China. The combination of solid phase micro-extraction with gas chromatography-mass spectrometer(SPME-GC/MS) has been widely used in the study of Volatile Organic Compounds(VOCs) secretted by the plants and microorganisms. But little information is available about the changes of VOCs in the system of rice straw biodegradation, especially the relationship between the changes of VOCs and the degradation mechanism of rice straw. Fourier Transform Infrared Spectroscopy(FTIR) is a supporting technology to track the changes of functional groups thus deduce the corresponding compounds. It has been widely used for analyzing the chemical changes in lignocellulose during decay or chemical treatments. However, the FTIR spectroscopy information on the degradation of rice straw in mixed culture of fungi, such as Phanerochaete chrysosporium and Trichoderma viride, is still limited.In this study, Phanerochaete chrysosporium and Trichoderma viride were inoculated in rice straw powder to make the mixed culture, and the pure cultures of them were set up respectively. Three aspects of analyzation and comparison was carried out among the three culture systems.The study contents includes the changes of the VOCs, the chemical changes of the rice straw, and the activity of Li P and Mn P. The results showed that: Trichoderma viride and Phanerochaete chrysosporium both degrade the easily biodegradable organisms in rice straw, such as waxes, cellulose and so on. Around the day 10, when the easily biodegradable organisms was insufficiency, both Trichoderma viride and Phanerochaete chrysosporium began to degrade the ligin in the rice straw to obtain nutrient. Although the Trichoderma viride replaced the Phanerochaete chrysosporium in the group C(the mixed culture) under the macroscopic observation, its degradation mechanism was different from the pure culture of Trichoderma viride. For the VOCs, the intensity of absorption band from FTIR and the enzyme activity detected in group C were all different from Group B. Morever, terpene(C10H16) was detected in the mixed culture, which might represent the antagonistic relationship between Trichoderma viride and Phanerochaete chrysosporium. That might represent the Phanerochaete chrysosporium still influenced Trichoderma viride in some way. The results from the detection of FTIR and enzyme activity showed that the group C possessed a better hydroxylation effect on lignin. But its ability in the degradation of aromatic rings was weaker than both the pure cultures of Trichoderma viride and Phanerochaete chrysosporium. That indicated the simultaneous inoculation of Trichoderma viride and Phanerochaete chrysosporium could improve the degradation effect of some components in the rice straw. But for other components, its effect maybe weaker than the pure inoculation. Therefore, it still need further research to investigate whether the simultaneous inoculation of Trichoderma viride and Phanerochaete chrysosporium could improve the quality of the composting overall.