Effects Of Dietary Riboflavin Level On Performance, Serum Biochemical Indices, Immunity And Antioxidant Function Of Growing Rex Rabbits

The purpose of this trial was to study the effects of dietary Riboflavin level on growth performance, serum biochemical indices and antioxidant function of growing Rex rabbits aged 3 to 5 months. One hundred and sixty-3-month-old Rex rabbits with an average were randomly assigned to 4 groups with 40 replicates in each group and each replicate contained 1 rabbit. Rabbits in control group were fed a basal diet, and rabbits in the experimental groups were fed experimental diets containing 0mg/kg、3mg/kg、6mg/kg、12mg/kg Riboflavin. Flowing are results of experiment:The result showed that dietary Riboflavin levels did not have significant influence s on daily intake(ADI), average daily gain(ADG) and feed/gain(F/G)(P>0.05). Ribofl avin of rabbit dietary were not changed PH and b*, but it have effects on L* and dri age in muscle(P<0.05), and had a significant effect on a*and drip loss in rabbit mu scle(P<0.01), fur thickness and wool length(P>0.05), while adding riboflavin had si gnificant effects on fur area and fur weight(P<0.05). Dietary riboflavin affected folli cle density and s/p(P<0.01). Dietary riboflavin had significantly affected on primary follicle and the secondary follicle density. Dietary riboflavin did no affect on the level s of growth hormone、t3、t4 and insulin(P>0.05). Dietary riboflavin had a significa nt effect on the contents of cholesterol(CHO)(P<0.05), however, it did not significan tly affect the contents of serum total protein(TP), albumin(ALB), triglyceride(TG),high density lipoprotein cholestorol(HDL-C) and low density lipoprotein cholestorol(L DL-C)(P>0.05). Thymu and thymus index were affected by dietary riboflavin(P<0.05),while spleen and spleen index were not had effects(P>0.05). The quantity of M cell in sacculus、immunoglobulin G、immunoglobulin A and il-2 were significantly affected by dietary riboflavin levels(P<0.05), but had no effects on immunoglobulin M(P>0.05). Dietary riboflavin level had effects on serum lutathione peroxidase(GSH-Px) activity(P=0.0328), and had significantly affects on liver GSH-Px activity. Riboflavin s upplemental level did not significantly affect serum and liver total superoxide dismutas e(T-SOD)、malondialdehyde(MDA) content and liver total antioxidant capability(T-AOC)(P>0.05). Reduced glutathione(GR) and methemoglobin(Met Hb) were affected by rib oflavin level(P<0.05), at the same time, riboflavin level had have significant influen ces on glucose 6-phosphatedehydrogenase(G-6-PD)(P=0.0002), but had no effects on gultathione-S-transferases(GSTs)in liver(P>0.05). Riboflavin level had no influences on gultathione-S-transferases(GSTs)、L-Glutathione(T-GSH)、reduced glutathione(G SH), while it had effects on Reduced glutathione(GR) and oxidized glutathione(GSS G)in liver(P<0.05). Riboflavin level had effects on transcription levels of reduced g lutathione(GR) and short-chainacyl-coadehydrogenasne(SCAD), but had no effects on gultathione-S-transferases(GSTs) transcription level. Considering all indices of this exper iments, 8.35mg/kg was the appropriate riboflavin level.