Research On Mass Rearing And Applying Chouioia Cunea Yang

Chouioia cunea Yang was an important parasitic natural enemies of Hyphantria cunea(Drury) and other forest pest insects. Based on reasonable planning and designing the breeding function room and developing a group of breeding and releasing apparatus, some key technologies on mass rearing including population rejuvenation and preservation, chosen of substitute hosts, inoculation proportion and inoculation method, cultivation and release were tested in this paper, which may optimize the breeding process and improve the breeding efficiency and success rate. The results were as following:1. Numerous breeding apparatus such as drawer-type homemade breeding box, inoculation box and the paper bag for releasing were independently designed and produced, which improved the breeding efficiency and success rate.2. Three approaches that achieving the population source of both H. cunea and C. cunea were proposed including seting aside insect-source site, dispersive collection and supplementary collection from nonlocal places. Two ways of population rejuvenation and preservation, the underground burial and overwintering in the field, were defined whose preservation rates were 94.7% and 93.9% respectively.3. The classification standard of tussah pupa was proposed for the first time. Generally, according to the pupa weight the tussah pupa could be divided into 3 leveles: small(6 ~ 9.0g), medium(9.1 ~ 12.0g), large(12.1 ~ 15g). For each level, the perfect inoculation ratio of C. cunea were 70:1, 100:1, 140:1 separately and the parasitic rates was all over 90%.4. Long-term storage technology of C. cunea was resolved. The study confirmed that the suitable morphology characteristics for long-term storage were old mature larva or a small amount of pre pupa. Keeping for 160 days at a temperature of 10℃and a relative humidity of 60 to 80%, and then slowly raising the temperature to 25℃, at this constant temperature condition, most of the adults of C. cunea began to eclose after 15 days and 94.4% adults complete eclosion in 24 days.5. Using C. cunea to control H. cunea had a certain effect, but it was not suitable at 2 ~ 3 generations of H. cunea. After releasing, the parasitic rate of C. cunea reduced with the decreasing of population quantity of H. cunea.