The Effect Of GnIH On The Reproductive Performance Of Female Goose And The Reproductive Development Of Rooster

Gn IH decreasing synthesis and release of gonadotropin applied to the anterior lobe of the pituitary gland or Gn RH. It is one of the most important reproductive hormone of animal reproductive performance regulatory. Nowadays, reproductive immune regulation is widely used to improve animal fertility. Many studies showed that applications of inhibin, prolactin, Gn IH and other reproductive hormones in vivo or in vitro can regulate animal growth and reproduction. Therefore, this experiment is mainly studied the regulatory mechanism of Gn IH with two different giving methods in reproductive development and reproductive activity of Magang geese and male chicken.In experiment 1, Selected 36 healthy adult female geese(1:5~6 with male goose) and divided into 3 groups(n=12). The first group(P group) was administered 1 mg Gn IH which is dissolved in 30% PVP-K30 in d1; the second group(G group) was administered 1mg Gn IH which is dissolved in saline in d1, d4, d7; and the third group( D group) was administered 1 m L saline in d1, d4, d7. During the experiment, we recorded the number of eggs and nests of the three groups every day. And blooded in d1, d4, d7 and d10 for measuring the hormone levels. At the end of the experiment(d10), kill the geese, measure their follicular development and collect hypothalamus and pituitary for detecting gene expression levels. The results showed that the laying geese which was administered Gn IH, to some extent inhibited the expression of Gn IH m RNA, and inhibited the laying performance, which may be mainly inhibits the development of LWF which is developing into SYF and WYF.In experiment 2, 36 healthy adult female geese(1:5~6 with male goose), which were in nest period, were randomly divided into immunized group and control group(n=15). At the beginning of the test, the geese were forced to terminate the nest artificially(7~10 days). Immunized the geese in chest muscle on days 1, 21, 41 from the test. The immunized group was each administered with 1m L volume on days 1, 21, 41(contained 1 mg,1 mg,0.5 mg Gn IH immunogen). The control group was each only administered with 1m L mineral adjuvant on days 1, 21, 41. The geese were exposed to short photoperiod(11L:13D) at the beginning to maintain their normal production. On days 72 Daily photoperiod was extended to 18 hours(18L:13D) and maintained to days 95. During the experiment, we recorded the number of eggs and nests of the three groups every day. Collected the blood samples on days 1, 21, 41, 61, 95 to measure the hormone levels. And killed the geese(n=8), measured their follicular development and collected the hypothalamus and pituitary for detecting gene expression levels. The results show that immunized geese generated antibody against Gn IH, inhibited the expression of Gn IH m RNA, and slightly increased the m RNA expression of Gn RH, VIP, PRL. Then regulated the reproductive hormones in serum and improved the laying of geese. In addition, photoperiod extended inhibited the laying of geese. But the effect on the control group were stronger. It may be because the auxo-action of Gn IH immunogen made in the immunized group was still maintaining.In experiment 3, 120 healthy roosters(60days old) were randomly divided into immunized group and control group(n=60). Immunized the roosters in chest muscle on days 1, 21, 41 from the test. The immunized group was each administered with 1 m L volume contained 0.5 mg Gn IH immunogen. The control group was each only administered with 1 m L mineral adjuvant. During the test, we collected the blood samples every 20 day from 60 d in vein under the winds to measure the antibody and hormone levels. Respectively selected 8 roosters from each group and slaughtered them to collect their testicular tissue and detect the development of them. We began to training roosters artificial insemination(massage) from d195, and establishing conditioned reflex after a week. We detected semen quality every three days for five times. The results show that immunized roosters generated antibody against Gn IH and remained a high level during the entire experiment. Immunized Gn IH inhibited the expression of Gn IH m RNA, and increased the expression of VIP, PRL and LH m RNA. Then promote the development of testis and the secretion of testosterone, INH, ACT in blood circulation. It also improved the semen quality.