| The subject aimed to study the influence on different DE and protein levels diets of pregnant rabbit does on reproductive performance of females and production performance and health of pups. It provided a theoretical basis for the nutritive index of pregnant rabbit doe’s diet.Select 100 healthy and multi-parity New Zealand rabbit does which had similar body condition, were randomly divided into 5 groups and 20 replicates of each group. After pregnancy, each group of rabbit does were fed the appropriate quantitative diet with standard diet (standard group DE 10.33 MJ/kg, CP 15.54%), low DE and low protein diet (DE 9.3 MJ/kg, CP 13.44%), low DE and high protein diet (DE 9.4 MJ/ kg, CP 18.63%), low DE and high protein diet (DE 11.15 MJ/kg, CP 13.74%), high DE and high protein diet (DE 11.18 MJ/kg, CP 18.83%) to childbirth. Rabbit does were fed and managed by the common rule of farm after delivery to weaning to measure the related indicators of reproductive performance. Rabbit pups were take care according to regular feeding and management after birth to 60 days, and measured the productive performance of 33-60 days. Selected 10 weaning rabbits which close to the average weight of the group and healthy of each group for the injection of lipopoly-saccharide, building intestinal injury model to test the performance of the intestinal mucosal immune of pups.Test results are as follows:(1) During the pregnancy, the rabbit does were fed different DE and protein levels diets would have significant effect on reproductive performance. There were no significant difference on serum follicle stimulating females hormone, luteinizing hormone and the number of birth and live children of different nutritional levels (p> 0.05). Serum estradiol levels in females, low DE low protein were significantly or very significantly higher than the other groups (p<0.05 or p<0.01). Females serum insulin-like growth factor-I content, the high protein groups (low DE and high protein, high DE and high protein group) were significantly higher than the standard group and the was standard group significantly higher than the low protein groups (low DE and low protein, high DE and low protein group) (p< 0.01), The rabbit does’serum prolactin level and born alive child, the high DE and high protein group, the high DE and low protein group, the control group and the low energy and high protein group were all significantly more than the low energy and low protein group (p< 0.05). The result showed that newborn litter weight, birth weight,21-day litter weight, weight of weaning litter, weaning weight were all in very significant difference, the high DE and high protein group was the largest (p< 0.01). The survival rate of the high DE and high protein group weaned pups was higher than the rest of four groups, but there was no significant difference (p> 0.05).(2) The dietary of different DE and protein levels to feed the pregnancy of rabbit does had significantly influence on intestinal mucosal immunity performance of their pups. The size of lymphoid nodules, IL-6 and SIgA of the weaned pups intestinal mucosa is extremely significant influence by the maternal nutrition, high DE and high protein group was significantly or very significantly higher than that of low DE and high protein group (p<0.05 or p<0.01), while low DE high protein group was significantly high in the other three groups (p<0.01). The IFN-γ, TNF-α, PlgR of the weaned pups intestinal mucosa were not significantly different between high DE and high protein and low DE and high protein group (p>0.05), but these two groups were significantly higher than the other three groups (p<0.01). The low DE and high protein group was significantly higher than the other four groups of the TLR4 (p< 0.01). The weaned pups intestinal mucosal TLR4 and P1gR mRNA expression was significantly affected:TLR4 with low DE and high protein group was significantly higher than the other four groups (p<0.01), P1gR high DE and high protein group was significantly higher than the other four groups (p<0.01), the results of mRNA expression were substantially the same as protein concentration in the mucosa.(3)The production performance and health of weaned rabbits were significantly affect by there mothers’nutritionist during pregnancy. The weaning trial rabbit initial weight of each group were significantly different, especially the high DE and high protein group was significantly higher than the other four groups (p<0.01), so to its final weight and ADG. While ADFI and F/G were not significantly different affect by the maternal nutrition (p> 0.05). The high DE and high protein group was the highest and the high DE and low protein group was lowest of diarrhea, mortality and health risk index.In summary, improving the nutritional levels of diets during pregnancy could improve birth weight and milk yield, thereby increase weaning weight and daily postweaning gain. It is also could significantly increase the concentration of IL-6, TNF-a, P1gR and ileal mucosal lymphoid nodules size of there pups’intestinal mucosa which might increase the synthesis and secretion of SIgA, besides increase the concentration of TLR4, which could improve intestinal mucosal immune performance and health of pups after weaning. The experiments showed that the effect of protein level was significantly higher than DE of the pregnant rabbit does and there pups. So the protein is more important than DE during pregnancy, but high energy high protein is the best. |
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