Studies On The Tissue Culture Of Xanthoceras Sorbifolia

In this paper, to study the effect on the callus differentiation,adventitious buds induction and rooting in different growth regulating substances, we chose stem segments, mature embryos and leaf of Xanthoceras sorbifolia as explants. The paraffin section showed the process of cytological study in tissue culture. The results showed that:1.The survival way to disinfect stem of Xanthoceras sorbifolia was5%NaClO drip axils+75%alcohol30s+0.2%HgCl28min (add2drops of Tween-20)+75%alcohol30s,the survival rate was52.78%, the contamination rate was31.94%, and the mortality rate was15.28%; in the trial of adventitious buds induction, we can get adventitious buds on the top of stem, and the growth of the adventitious buds was excellent;the stem segments which was cultured in67d can get12.10buds on averagein in subculture.2. In this cultrue, the suitable explant was mature embryos,and the suitable medium for adventitious buds induction in initial culture was MS+6-BA2.0mg.L-1+NAA0.1mg.L-1+sucrose30g.L-1+agar6g.L-1,and the differentiation rate was94.7%;the suitable medium for adventitious buds height growth in subculture was MS+6-BA0.5mg.L-1+NAA0.5mg.L-1+sucrose30g.L-1+agar6g.L-1,then50d later,the average seedling height (>0.05cm) was11.67buds; the suitable medium for rooting culture was WPM+IBA1.0mg.L-1+sucrose30g.L-1+agar6g.L-1, and the rooting rate was70.30%, the average root number was3.57. The paraffin section showed the process of callus induction, differentiation of callus and adventitious buds development.3. In this cultrue,the suitable explant was spears,and the suitable medium for non-embryogenic callus induction in initial culture was B5+KT1.0mg.L-1+2,4-D1.0mg.L-1+sucrose30g.L-1+agar6g.L-1+lactoalbumin hydrolysate400mg.L-1; the suitable medium for embryogenic callus induction in initial culture was B5+KT2.0mg.L-1+IAA2.0mg.L-1+sucrose30g.L-1+agar6g.L-1+lactoalbumin hydrolysate400mg.L-1;in suspension culture,we can get the callus like:subsphaeroidal callus, clavate callus, irregular callus; in solid culture with agar,we could get seedlings and buds.