| Salix psammophila is the pioneer trees of afforestation and wind prevention and sand fixation. In this paper,put the Salix psammophila different explants, preliminary study the tissue culture of rapid propagation system and regeneration system. The study is a significance work to carry out research on Salix psammophila genetic engineering by using biological technology.The main research results are as follows:(1) disinfection methods:analysis from the three kinds of explants disinfection methods,75% alcohol lmin+10% sodium hypochlorite 5min explant survival rate was the highest, up to 43.3%,for the best of Salix stem explants disinfection method.(2) ZT, NAA hormone combination on the effects of culture of Salix twig tissues:in the use of ZT and NAA hormone combination, the sterile shoots growth of suitable culture condition for 1/2WPM+ZT1.0mg·L-1+NAA0.1mg-L-1+PH5.8 was up to 1.7cm, the sterile shoots rooting culture conditions suitable for the growth of 1/2WPM+ZT0.1mg·L-1+NAA2.0mg·L-1+PH5.8 up to 80%.(3) 6-BA and NAA hormone combination on the effects of culture of Salix twig tissues:it found that the suitable culture conditions induce high growth of shoots was WPM+NAA2.0 mg·L-1 was up to 4.3cm;the suitable culture conditions induce shoots rooting was WPM+NAA0.1mg·L-1+PH5.8,up to 100%the suitable induction conditions of Salix twig lateral bud growth was WPM+NAAlmg·L-1+PH5.8,up to 1.8cm.Lateral bus growth is highest for 1.8cm.NAA had significant promoting effect on the growth of shoots, but 6-BA shoots growth play a significant inhibition.By the analysis influence of growth of ZT, NAA and 6-BA, NAA two h ormones combination of salix shoots, he study found that 6-BAã€NAA hormone combination is better than that of ZT, NAA hormone combinations, more suitable for the growth of salix shoots, roots, etc.(4) Effect of different basic media on the tissue culture:using WPM, MS, DCR, White, B5 and other 5 kinds of basic culture medium, the study found that the best culture medium on the growth of the shoots was WPM.(5) PEG-6000 inhibited the growth of Salix twig water stress:the study found that, with the PEG-6000 content in the culture medium of callus increased, can be formed by the salix twig to base, but the callus diameter decreased from 0.69mm to 0.4mm, the root length from 0.6cm to 0.32cm, decreased significantly; in different PEG-6000 content in shoots, Salix can germinate bud height, but with the increase of the concentration of PEG-6000 decreased, the growth of axillary bud height decreased from 0.54cm to 0.19cm. The results showed that PEG-6000 simulated water stress had inhibitory effects on callus formation and bud growth of Salix twig.(6) Study on regeneration system of Salix stems:this experiment used cut stems as explants, hormones of 6-BA and NAA combination, the stems, the callus induction rate of the culture conditions suitable for 1/2WPM+6-BA2.5mg·L-1+NAA0.5 mg·L-1,up to 100%; culture conditions suitable for the tender branches and stems callus increment 1/2WPM+ 6-BA0.5mg·L-1+NAA1.5mg·L-1,up to 5.5mm; the longitudinal stem rooting rate of Salix psammophila culture conditions suitable for 1/2WPM+6-BA0mg·L-1+NAA0mg·L-1, the root length of up to 100%.(7) Study on regeneration system of Salix leaves:The callus induction rate of Salix leaf culture conditions suitable for 1/2WPM+6-BA1.5mg·L-1 and the induction rate was 100%; the value of Salix leaf callus culture conditions suitable for 1/2WPM+ 6-BA1.0mg·L-1+IBA0.5mg·L-1, up to 2.7mm;the suitable for adventitious buds is 1/2WPM+6-BA2.0mg·L-1+IBA 1.0mg·L-1, up to 40%.In the choice of salix slitting stem section and tender leaves as explant reg eneration system study, found that the leaf is suitable for adventitious bud induction, and longitudinal cutting stem section is suitable for callus proliferation. |
