Studies On The Main Extract Ingredient And Shrimp Lethal Activity Of Phyllostachys Praecox

In this thesis, Phyllostachys praecox in Zhejiang has been used as object of study.After the extraction with methanol, the extract (A0) had been concentrated, and then addedwater to make it suspension. Using petroleum ether, ethyl acetate and n-butanol extract thesuspension successively. After concentrating the three extracts, petroleum ether phase (A1),ethyl acetate phase (A2) and n-butanol phase (A3) has been got. Through separation andpurification of A2twelve monomeric compounds: YLW-1~YLW-12, and seven of twelvecompound has been identified by MS,1H NMR and13C NMR, the seven monomericcompounds is tricin, β-sitosterol, daucosterol,4-aminobenzaldehyde,2-hydroxy-4-methoxybenzaldehyde, friedelin and lupeol. The above compounds were separated fromPhyllostachys praecox for the first time and4-aminobenzaldehyde and2-hydroxy-4-methoxybenzaldehyde were separated from Phyllostachys for the first time.Shrimp lethal activity experiments of A0, A1, A2, A3, daucosterol, tricin and friedelinhave been done to investigate their anti-tumor activity. Their lethal concentration (LC50):LC50(A0)=0.057g/mL； LC50(A1)=0.045g/mL； LC50(A2)=0.053g/mL；LC50(A3)=0.070g/mL； LC50(tricin)=0.045g/mL； LC50(daucosterol)=0.010g/mL； LC50(friedelin)=0.068g/mL。...