Collection And Rapid Propagation Of Several Lilium Resources In Yunnan Province

Lily is one of perennial herb plants belonging to the genus Lilium of Liliaceae family. As one of the most important cut flowers, lily is widely distributed and has multiple diversities. China is rich in wild lily resources. Yunnan, Sichuan and Guizhou are richer in wild lily resources than other provinces. As one kind of flowers with high ornamental, medicinal and nutritional value, lily is largely explored. The survey reports of recent years showed that many wild lilies with ornamental value and endemic species of China are endangered. In order to solve the problem, I did the research on the lily resource in Yunnan Province. Besides, the original habitats of Lilium bakerianum var. aureum, L. taliense and L. sulphureum have been investigated. Base on the previous research, I have also researched on regeneration and propagation system which can be applied to the protection of resources of Lilium in China. The main conclusions can be drawn as follows:1. Four species of wild lily in Yunnan Provinces including L. taliense, L. duchartrei, L. bakerianum var. aureum and L. sulphureum were collected in this study. Their habitats, plant communities, biological characteristics and ornamental characteristics were surveyed though sample plot survey. They are distributed primarily on the mountains of southwest China. L. bakerianum var. aureum usually grows at steep slope, in the forest edge and at the altitude of2680-2880m. It is rarely found and has yellowish flower with purple spots. L. taliense grows at gentle slopes, at fascine dam and at the altitude of2998-3013m. L. taliense is random distributed and has white flower with purple spots which is of high ornamental value and nutritional value. L. duchartrei grows at open gentle slopes and at the altitude of3143-3152m. They are clustered together and have pink flowers with purple spots. L. sulphureum grows on steep slope and at the altitude of1613to1679m. It is randomly distributed and has large flowers which have rich aroma. Their low reproduction rate and humans’ demolishment lead to the shortage of wild lily resources.2. The scales, bulbils and floral organs were used as explants in initial culture. The results showed that MS+6-BA1.0mg/L+NAA0.3mg/L was suitable for the initial culture of ovary and MS+NAA0.1mg/L was suitable for most explants which had strong differentiation abilities. In all the explants, bulbils have the highest survival rate. The results showed that the survival rates are highest by using2%NaClO solution to deal with the scales of L. bakerianum var. aureum for20min,2%NaClO solution to deal with the scales of L. duchartrei for15min and0.1%HgCl2solution to deal with the bulbils of L. sulphureum for15min.3. The research used tissue culture plantlets of L. sulphureum as renewable materials to establish an efficient propagation system. The results showed that MS+sugar40g/L+6-BA0.5mg/L+NAA0.5-1.5mg/L+TDZ0.1-0.2mg/L was optimum for the regeneration of adventitious buds. The best culture medium for regeneration of bulblets was MS+sugar30g/L+2,4-D0.1-1.0mg/L. MS+6-BA0.5mg/L+NAA0.1mg/L was suitable for propagation of bulblets leaves and every bulblet leaf can produce7.6little bulblets.3MS+sugar50g/L+activated carbon1-3g/+NAA0.1mg/L+CCC15mg/L/choline chloride100-150mg/L/PP3335mg/L/ABA1.0mg/L was suitable for the enlargement of bulbs.