Research On The Effect Of Low Temperature Induction On Related Physiology Biochemistry Of Pleurotus Ferulae Primordium Formation

The Pleurotus ferulae is a kind of edible mushroom which could forming primordium and fruitingbody only after low temperature induced process. Low temperature induced process is key of vitalimportance for the Pleurotus ferulae cultivation and getting economic benefits, so masting the temperaturefor primordium formation is a necessary technology of artificial cultivation. This study used Pleurotusferulae5.0774as experimental strain. By controlling the induced temperature and time, effect of lowtemperature treatment condition on primordium formation was determined. At the same time, myceliumassociated extracellular enzymes secretion, matrix degradation ability and antioxidant enzyme activity threeaspects were researched in order to reveal physiological changes of Pleurotus ferulae mycelium duringcryogenic treatment. The main results are as follows:(1) Primordia were found in the treatment5℃,10℃for25d and30d. Considering the perspective ofprimordium formation days, numbers and rate, the optimum low temperature treatment conditions ofPleurotus ferulae primordium differentiation was10℃for25d.(2) Low temperature treatment caused the enzyme activity changes of CMC, HC, amylase, laccase andproteinase. The activity of CMC enzyme, HC enzyme and acid protease decreased after low temperaturetreatment. The activity of laccase, amylase and neutral protease were significantly higher at the early stageof the low temperature treatment. Laccase and neutral proteinase activity increased, acidic protease activitydecreased after a certain time and intensity of the low temperature treatment. Then accelerated the originalsubstance decomposition, small molecules of carbon and nitrogen substrates were prepared for thesynthesis of new substances, induceing primordium formation.(3) In the cryogenic processing mycelium can degrade all components of lignocellulose in mediumwithin the low temperature processing of30d. The cellulose, hemicellulose and lignin degradation rate of5℃and10℃treatment group were significantly higher than in15℃treatment group and25℃control group. During this period, the degradation of cellulose and lignin had certain advantages, priority use ofhemicellulose, followed by lignin and cellulose.25℃control group priority used of hemicellulose andcellulose, lignin degradation was less.(4) The results of study on antioxidase activity in Pleurotus ferulae mycelia showed that there wassignificant difference in the activities of SOD, CAT and POD between the low temperature treatment groupand control group. SOD and CAT activity increased at the early stage of the treatment and always higherthan that of the control. POD activity decreased during low temperature treatment and the level is lowerthan the control group. It showed that when environment temperature decrease the Pleurotus ferulaemycelia would make some appropriate response and SOD and CAT were involved in low temperatureresponse. Higher SOD and CAT activity helped mycelia to acclimatize low temperature.