Effect Of Low Temperature Treatment On Physiological Biochemical Indexes And Nutrient Accumulation In Pleurotus Ferulae Mycelia

Pleurotus ferulae, which grows in the arid steppe, are low temperature ediblefungi. The fruiting body formation must be cold stimulated, but the mechanismremains unclear, the current research on its mechanism of cold-induced fruiting arenot too much. In this paper, the Pleurotus ferulae (NB-1) was used as liquidfermenta,25℃oscillation cultured for7days,10℃low temperature processing3h,6h,9h,12h,24h,36h,48h. The non-denaturing polyacrylamide gel electrophoresis(PAGE) technology was used to determine the laccase, tyrosinase isoenzymechanges of the myceliumhe mycelium superoxide dismutase (SOD), catalase (CAT),laccase, tyrosinase activity changes as well as the the Pleurotus ferulae myceliumamino acids, protein, vitamins accumulation under low-temperature processing, themain findings are as follows:(1)1×PBS: mycelium (v/w)1:1had a better effect than other methods on theextraction of the protein in the Pleurotus ferulae mycelium. The molecular weight ofthe low temperature treatment group>98KDa protein was significantly less than thatof the control group (25℃), this phenomenon at low temperature (10℃treatmentfor3h to12h) were obvious; When the cryogenic processing time was longer than12hs, the bands (molecular weight>98KDa)were increased, but still less than that ofthe control group.(2) After low-temperature treatment, the bands of laccase and tyrosinaseisoenzymes of Pleurotus ferulae mycelium were changed. Cold treatment, a bar(molecular weight of the28.0KDa of tyrosine isoenzyme fades, and a band (themolecular weight of43.5KDa) was significantly deeper than the control group. Afterlow-temperature treatment, laccase isoenzyme molecular weight of two of45.0KDaand30.0kDa enzyme with significantly deeper, and late in the low-temperaturetreatment (treatment for24hs to48hs) emerging enzyme with a molecular weight of33.0kDa. (3) Under10℃low temperature treatment SOD, CAT vitality ofPleurotusferulae mycelium were higher than10℃treatment within3hs to48hs, laccaseactivity increased significantly (p <0.01), within9h-12h, the laccase activitysignificantly improved, laccase activity changes stabilized after treatment for24hours.10℃treat3hs to12hs, the tyrosinase activity were lower than the controlgroup. But when10℃treat12hs to48hs, tyrosinase activity was significantlyincreased, and higher than that in control group.(4) After low-temperature treatment,10℃for6hs had the highest essentialamino acids and total amino acid c.After low temperature treatment, the amount ofthe amino acids was higher than the control group, and the highest total amino acidwas in10℃treat6hs,and it was2.32times that of the control group. Thesulfur-containing amino acids methionine and cystine were the limiting amino acidsin Pleurotus ferulae mycelium.