Effect And Mechanism Of Graphene Oxide On Cymbidium Hybridum Tissue Culture

To probe the method about the efficiency of micro-propagation and its quality improvement for Cymbidium hybridum seedlings, the paper systematically studies the effect of Graphene Oxide on the abduction, proliferation, differentiation and the growth of tube seedlings of Cymbidium hybridum protocols. At the same time, it discusses the mechanism of anti-browning of the induction training process for the original bulbs in order to provide proof for establishing the system of the rapid reproduction technology for Cymbidium hybridum.The main results of research in this thesis are followed:l.The best recipes medium at different stages of tissue culture of Cymbidium hybridum:①Best recipes medium of iduced protocorm:MS+6-BA1.0mg/L+NAA0.2mg/L+harose30g/L+agar7g/L+GO0.05g/L,the induction rate could be88.3%after45d.②Best recipes medium of multiplication Protocorm:MS+6-BA2.0mg/L+NAAO.5mg/L+saccharose30g/L+agar7g/L+GO0.05g/L, the multiplication factor could be2.0afer45d.③Judging from the differentiation and browning rates, the low concentrations of activated carbon is better than graphene oxide in culture of bud differentiation. So the best recipes medium of bud differentiation is MS+6-BA2,0mg/L+NAA0.5mg/L+saccharose30g/L+agar7g/L+AC0.5g/L,the differentiation rates could be400%afer45d.④Whether bud height, weight, number of leaves,adding low concentration of culture medium at the bud stage of graphene oxide over activated carbon.So the best recipes medium of bud high is MS+6-BA1.0mg/L+NAA1.0mg/L+saccharose30g/L+agar7g/L+GO0.05g/L,the bud growth index could be3.14after45d.⑤The medium of rooting:1/2MS+saccharose30g/L+agar7g/L+GO0.05g/L,the rooting rate could be100%after45d,Stem diameter, root length.⑥Comprehensive factors of seedling index, seedling height, leaf number,the best recipes medium of sound seedling is MS+6-BA1.0mg/L+NAA0.5mg/L+sacchar ose30g/L+agar7g/L+AC1.0g/L. ⑦.Studies have shown that the degree of browning for explants is reduced by decreasing the TP, PPO and PAL of training materials as well as by adding AC and GO with different concentrations during the induction training process for the original bulbs. The effect of such treatment is better when the concentration of AC is0.5g/L and GO is0.05g/L.