Effects Of Feed The Tea Polyphenols And Lactobacillus On Physiological And Intestinal Function In Broilers

Lactobacillus is one of probiotic bacteria,polyphenols is a natural beneficial substances extracted from tea,they have the lots of functions.To explore the effects of lactobacillus,polyphenols,and a combination of both on animal physiological functions,the study chosed four hundred and eighty broilers were selected and randomized into eight groups, there are sixty broilers in each group which is consisted of six replicates, that is ten broilers in each replicate was setExcept for giving basic feed, the test groups were given lactobacillus and tea polyphenols by gavaged while the control group was given sterile water on the same condition The whole experiment lasted for56days.The test results of growth performance,blood biochemicalparameters,digestive enzyme activities,and intestinal microbial diversity index showed that:First,growth performanceThe broiler’s body weight of lactobacillus group’s by gavaged than than control group were reduced25.52%(P<0.05),the average daily gain were reduced6.30%(P>0.05),the average daily feed intake were reduced21.69%(P<0.01),and the feed conversion ratio were improved32.42%(P<0.01),the broiler’s the average daily gain of polyphenols group’s by gavaged than the control group were improved7.22%(P>0.05),the average daily feed intake were reduced7.13%(P>0.05), the feed conversion ratio were improved reduced6.64%(P>0.05), body weight were improvedl.28%(P>0.05),1.0[ml/(kg.bw)] lactobacillus plus0.06[g/(kg.bw)] polyphenolgroup’s effect on growth performance of is most visible in the eight groups.Second,blood biochemical parameterBy gavaged lactobacillus group’s improved broilers of concentrations of LDL-C> GLU, TG were respectively reduced24.21%(P<0.01)、16.27%(P<0.01)、25%(P<0.01) than the control group, polyphenols group’s by gavaged improved broilers of concentrations of LDL-C、GLU、TG were respectively reduced28.26%(P<0.01)、28.99%(P<0.01)、16.27%(P<0.01),1.0[mL<kg.bw)]lactobacillus plus0.06[g/(kg.bw)] polyphenol group’s by gavaged reduced broilers of concentrations of TG、LDL-C、CHO, and Lactobacillus and polyphenols have interaction reduced blood biochemical parameter. Third,digestive enzyme activitieCompare with the control group, by single gavages lactobacillus improved activity of the lipase of small intestine and pancreas in broilers were respectively increased24.32%(P>0.05)、7.81%(P>0.05); activity of pepsin of gizzard stomach and glandular stomach in broilers were respectively increased17.12%(P<0.01)、44.16%(P<0.05), activity of the trypsin of small intestine and pancreas in broilers were respectively increased29.17%(P<0.01)、27.78%(P<0.05),activity of the amylase of small intestine and pancreas in broilers were respectively increased18.52%(P<0.05)、1.30%(P>0.05); by single gavages polyphenols improved activity of pepsin of gizzard stomach and glandular stomach in broilers were respectively increased13.65%(P<0.01)、55.84%(P<0.01), activity of the lipase of small intestine and pancreas in broilers were respectively increased11.93%(P>0.05)、13.98%(P<0.05), activity of the trypsin of small intestine and pancreas in broilers were respectively increased8.33%(P>0.05)、33.33%(P<0.05),activity of the amylase of small intestine and pancreas in broilers were respectively reduced7.41%(P>0.05)、7.79%(P <0.05).Lactobacillus and polyphenols have interactionor repel,and combined use has more favorable impact than single gavages.Among those combinations, the lactobacillus middle dose group’s affected on digestive enzyme activities of56-days-old broiler is the invisible,1.0[mL/(kg.bw)] lactobacillus plus0.06[g/(kg.bw)] polyphenol group’s activity of the lipase and the trypsin of small intestine, pepsin of gizzard stomach and glandular stomach, the trypsin of pancreas, the amylase of small intestine and pancreas by gavagedthan by single gavages polyphenol were respectively increased42.39%(P<0.05)、123.08%(P<0.01),18.78%(P<0.01),30.62%(P<0.01),45.83%(P<0.01),34%(P<0.01)、11.27%(P<0.01).activity of the lipase of small intestine, pepsin of gizzard stomach and glandular stomach, the trypsin of small intestine and pancreasthan by single gavages lactobacillus were respectivelyincreased28.20%(P<0.01),15.25%(P<0.01)、41.20%(P<0.01),87.10%(P<0.01)、52.17%(P<0.01).Forth,intestinal microbialTest showsthat lactobacillus and polyphenol by gavaged groups all got richer biodiversity than the control group,when1.0[mL/(kg.bw)]lactobacillus plus0.06[g/(kg.bw)] polyphenol group’s by gavaged got the highest microbial intensive and the most dominant flora,there is advantage bacterium-lactobacillus crispatus strain; the0.09[g/(kg.bw)] polyphenol plus1.0[mL/(kg.bw)] lactobacillus group’s existence of dominant bacteria-pseudomonas veronii strain.When1.5[mL/(kg.bw)] lactobacillus plus0.06[g/(kg.bw)] polyphenol group’s existence of dominant bacteria-lactobacillus reuteri strain.