| In this study, a total of 337 individuals of excellent native goat breeds-Guizhou White Goat, Guizhou Black Goat and Qianbei Ma Goat, were chosen as research object. To obtain molecular genetic assisted marker for enhancing the growth traits of common individual of goat observably, the technology of DNA pooling, PCR-SSCP and direct sequencing were used to research the SNP of STAT5 A and STAT5 B gene, genetic structure, genetic diversity and association analysis with growth traits of different goat breeds. Meanwhile the expression level of STAT5 A and STAT5 B gene in 9 different tissues of Guizhou White Goat was analyzed by Quantitative Real-time PCR. The major experimental results obtained in my study were as follows:1 3 SNPs were screened in STAT5 A and STAT5 B gene respectively.2 The results of STAT5 A gene showed that, the C11447 T locus affected body height in Guizhou White Goat(P<0.05). The G14264 A locus had effects on body weight, body height and chest girth in Guizhou Black Goat(P<0.05). The G14320 A locus in Guizhou White Goat had effects on body length, hucklebone width and chest girth(P<0.05), and had an effect on body weight and chest girth in Guizhou Black Goat(P<0.05).3 The T16159 C locus in Guizhou White Goat had influence on body weight, body length, hucklebone width and chest girth(P<0.05), and on body weight, body height, hucklebone width and chest girth in Qianbei Ma Goat(P<0.05). The C20844 T locus in Guizhou Black Goat had influence on chest girth(P<0.05), and on body weight, body length, hucklebone width and chest girth in Guizhou White Goat(P<0.05). The C21455 T locus in Guizhou Black Goat had inluence on body weight and chest girth(P<0.05), on body weight, body height and body length in Qianbei Ma Goat(P<0.05), and all the five growth traits in Guizhou White Goat(P<0.05).4 The expression level of STAT5 A and STAT5 B gene in different ages of Guizhou White Goat was as follows:(1) The expression level of STAT5 A gene in 12-month-old Guizhou White Goat:liver> pituitary>heart> hypothalamus>lung> spleen > cerebellum >brain> kidney(2) The expression level of STAT5 A gene in adult Guizhou White Goat: heart> lung >brain> pituitary> hypothalamus> cerebellum > spleen >liver> kidney(3) The expression level of STAT5 B gene in 12-month-old Guizhou White Goat: liver>pituitary>heart> spleen > cerebellum > lung >brain> kidney > hypothalamus(4) The expression level of STAT5 B gene in adult Guizhou White Goat: heart> lung >brain>liver> pituitary> kidney > spleen > cerebellum > hypothalamus5 The expression level of STAT5 A and STAT5 B gene presented higher in liver and pituitary in 12-month–old Guizhou White Goat, and presented higher in heart, lung and brain in adult Guizhou White Goat, it suggested that STAT5 A and STAT5 B gene might the important genes which regulated the early growth in goats, and with the maturation of individuals, their main effects and working part changed, meanwhile the two genes had high coordination and consistency. |
