The Study Of Spermatogenesis And Seminiferous Performance Of Liaoning Cashmere Goat Ram

Liaoning cashmere goat not only has the highest cashmere production in the world, but also has the most extensive use of cashmere goat breed resources in our country. With the acceleration of the country producing cashmere goat breeding process, and how to improve the efficiency and utilization of reproductive potential of Liaoning cashmere goat ram has to break the current bottleneck problem cashmere goat healthy development of the industry, but the key to solving this problem is to understand Liaoning Cashmere goats and rams spermatogenesis age law triggered changes in semen quality and testicular germ cell how changes in the process and so on. In this study, respectively, of different ages, Liaoning cashmere goats for the study, through the Liaoning cashmere goat lambs and other major testicular development and spermatogenesis into reproductive organs, regulation of apoptosis and Bax gene protein Bcl-2 transcription and expression levels and semen quality Liaoning cashmere goats were studied and their use of the system. 1. lambs 0 to 180 continuous observation for 40 days between the date of birth is close to, and obtain the following results:(1) Liaoning cashmere goat lambs early sexual development, and improve various sex all appear within 15 ~ 30 d after weaning.(2) Liaoning cashmere goat lambs born at extremely rapid growth and development, body weight after the birth of a month-old is more than three times during the time of birth, and post-weaning weight gain to about 6 months of age nearly doubled.(3) The main genital Liaoning cashmere goat lambs showed a year-old age trend growth and rapid development, when the 6-month unilateral testis, epididymis unilateral penis length and a fine of 238 times the pipe diameter into newborn when 53-fold, 7.2-fold and 6.14-fold, with the weight of testicular growth and development of the most rapidly.(4) the number of spermatogonia Liaoning cashmere goat lambs testicles inside was gradually increased since the birth of the state, there has been a primary spermatocytes at 30 days, secondary spermatocytes occurs 60 days, 90 days appear sperm cells and sperm, 180 days when the number of sperm cells begin to decrease sperm begins to increase; Liaoning cashmere goat lambs at 4 months of age can collect the semen, but semen quality individual indicators are low, yet can be fertilized, but 5 months after the semen quality indicators have been close to the adult rams.(5) With the growth of Liaoning cashmere goat-day-old lambs, which secrete Serum testosterone levels also showed a gradual increase in the state, 30 days after secretion into the time of birth was 2.23 times, 5 times, 7.94 times, 10.41 times and 13.45 times and 16.16 times. Description Rams Rams sexual decisions and the use of performance species and life direction; Rams beginning with the age and weight-related; the important function of the testes to produce sperm and testosterone is. 2. Liaoning cashmere goats 8 months to 18 months, 18 months to 24 months, 36 months or more physical properties of different ages ram semen detection in seasons four seasons, obtain the following results: Ages ram semen volume and sperm motility were better than in spring and autumn, winter, spring, summer and winter by the superior, young ram sperm motility than adult sheep; sperm density increased with different ages Seasonal changes in sperm density was highest in autumn; Liaoning cashmere goat sperm abnormality rate of adult rams to fall to the lowest, acrosome integrity was highest in summer and the highest percentage of abnormal sperm Rams acrosome integrity lowest in spring winter and winter is in between; the same classic yolk Diluent and no animal dilution dilution method, saving the state at low temperatures, the greater the dilution of semen, sperm in vitro longer survival time; temperature more high, two methods at the same dilution ratio, the survival time of the sperm in vitro decreased. Classic yolk dilution dilution method, at the same temperature, the survival time of the sperm in vitro, with the increase of dilution gradually extended; but no animal-derived diluent dilution method, at the same temperature, sperm survival in vitro time, with increasing dilution gradually reduced. Shows young ram sperm motility than adult sheep, should be selected as a young ram ram and the best breeding season for the fall, get a higher pregnancy rate. 3. Using quantitative PCR method differences mRNA 2-month-old and 15-month-old ram Liaoning cashmere goat testicles Bax and Bcl-2 gene expression studies to obtain the following results: Liaoning cashmere goat testicles before and after sexual maturity, Bax mRNA and Bcl-2 gene, and the expression of Bax mRNA amount of the difference was significant(P <0.01), the expression of Bax gene after sexual maturity before sexual maturity 120-fold difference in expression of Bcl-2 gene mRNA was not significant(P> 0.05), the expression of Bcl-2 gene after sexual maturity is 1.4 times before sexual maturity, both before and after the illustrative mature cell apoptosis, but mainly after sexual maturity.