The Virus Elimination Of Psendostellaria Heteriphtlla By Shoot-tip Culture And Its Effect On The Yield Improvement

Pseudostellaria heterophylla (Miq) is a famous native herbal medicine in Fujian province, with its mosaic disease forming a major problem to its production for high yield and better quality. In this paper, in order to provide its production with a scientific foundation, a preliminary technology system including shoot tip culture, virus detection and virus-free seedling transplantation was established through applying tissue culture and virus-free technology on the shoot tips of Pseudostellaria heterophylla (Miq) from Zherong county of Fujian province. The results were as follows:1. In the shoot tip culture, as for the plantlet formation ratio and average height of seedlings, a MS medium with a combination of 0.5 mg/L NAA and 0.5 mg/L BA was selected for this experiment, with 30 g/L sucrose and 10 days of dark culture being considered best, moreover, A fiber carrier liquid medium supplemented with 0.5 mg/L NAA and 0.5 mg/L BA was suitable for the culture of Pseudostellaria heterophylla.2. In the subculture, the cross culture means with two types of MS media, one of which was supplemented with 0. 5 mg/L NAA and 0. 5 mg/L BA, and the other 1.0 mg/L NAA and 0.5 mg/L BA, was superior in prompting the growth and propagation of the plantlets to the continuous culture means using only one type of MS media supplemented with 0.5 mg/L NAA and 0. 5 mg/L BA.3. In rooting in vitro, as for the percentage of rooting, numbers and length of rooting of per plant, a 1/2 MS medium with 1. 0 mg/ L IBA and 1.0 mg/ L IAA was best for the experiment, with 30 g/L sucrose and 10 hours of exposure to light per day being deemed best.4. In the transplantation of seedlings, as for the survival rate of the transplantation, the ratio of mixed soil: sand: plant ash= 1: 2: 0.5 sprayed with a vegetative solution of 1/10 MS was the best.5. The twp methods (the ocular estimation and indictor plant) were used in the virus detection of the plantlets. The preliminary results indicated that the plantlets did not carry mosaic virus with exfoliation shoot-tip culture.6 In the culture test, the results of observation indicated that the growth of seedlings derived from stem-tips was better than those derived from seminal root, seed, stem segment in stalk length, breeding period and yield per plant.Factors impacting the seedling formation from shoot tip, the virus-free effect through shoot tip culture, and the biological basis for the yield increase of virus-free seedlings were discussed also. Finally, some views on the research of the culture of Pseudostellari a heterophylla in vitro and its application in the production were put forward.