| In this reaserch, four experiments were conducted to determine the exogenous metabolism glucose in weaning pigs. Based on the comparison of the accuracy of three methods measuring starch, it further studied the digestion and utilization of starch in four kinds of daily ration.Experiment 1: This experiment was designed to test the accuracy of comparison of three methods for determination starch. 6 kinds of standard substance consist of soluble starch and cellulose with different proportions were determined the content of starch by acid hydrolysis method(2008GB/T), anthrone colorimetry,enzymatic hydrolysis,respectively.The result showed that enzymatic hydrolysis is more accurate than acid hydrolysis(2008GB/T) and anthrone colorimetry when used to determine the content of starch.Experiment 2: 6(ABW5.48±0.61kg) crossbreed(Duroc×Landrace×Yordshire)21d weaning pigs were conducted to study the digestion and utilization of starch in basic ration, which would provide basic data for further research of piglet glucose nutrition. The results showed that the content of exogenous metabolizable glucose in basal daily ration is 472.78 g/kg, when the average daily feed intake is 284.48 g, the daily intake of exogenous metabolism glucose of the weaning pigs was 108.09 g.Experiment 3: 20(ABW7.06±0.09 kg kg) crossbreed( Duroc×Landrace×Yordshire)(25±2)d weaning pigs were selected to install the T-Cannula in terminal ileum. With corn, feed grade glucose powder as a source of carbohydrate, with four iso-energy and iso-nitrogen daily ration, four different kinds of MG daily ration for digestion experiment. Studied the four daily ration nutrient starch digestion and utilization of the law, and estimated the value of MG of four kinds of daily ration according to the preliminary test results. The test results showed that the daily ration I, II, III, IV content of metabolism of exogenous glucose is 254.52g/kg, 315.04g/kg, 451.21g/kg, 490.27g/kg.In daily feed intake for 300 g,group I,II,III,IV weaning pigs daily intake exogenous glucose metabolism were 68.83 g and 85.40 g, 128.8 g and 139.99 g.Experiment 4: Based on small intestinal digestion time processing factors by single factor design, divided into 1h,2h,4h,6h,8h total of five processing, in order to test two of the four kinds of diet for the substrate, through the stomach- small intestine two-step method for in vitro digestion of feed. In this study, the four body with the test method of measuring the correlation of four kinds of diet exogenous glucose metabolism, to establish a determination of exogenous glucose metabolism in vitro method, and the method for determining the content of several common feed piglets exogenous glucose metabolism, thought that establish a piglet new feed evaluation system based on the metabolism of glucose and glucose nutrition regulation technology of practical foundation. The test results showed that:(1) The optimal digestion time of starch digestibility measured by in vitro method was 6h.(2) The regression equation of starch digestibility measured by in vivo method and starch degradation rate measured by in vitro method with 6h is y=0.1374x+84.834(R2=0.8711).Thus, the estimate formula for fodder exogenous glucose metabolism measured by in vitro method is IDSG =(0.14K’+84.83)/0.9×BS.(3) Contents of fodder exogenous metabolic measured by in vitro method of the six piglets are corn corn(Xinjiang)741.10g/kg,(Jilin)667.41g/kg,the second-class wheat(Jingmen)632.22g/kg, wheat bran(Zhoukou) 396.40 g / kg, soybean meal(46%, Jiangsu) 93.25 g / kg,soybean meal(43%, Wuhan) 84.11 g / kg. |
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