| Heteroptera:Miridae always as concurrently control target of Helicoverpa armigera. Behind 90th of 20th century, along with the popularization of transgenosis insect-resistant cotton, ecological status of cotton insect had a large change, the population quantity of Helicoverpa armigera had clear abated, subordination insect-Heteroptera:Miridae rose to be the main insect in cotton field. To evaluate the resistance of transgenic cotton to the two main cotton Miridae(Apolygus lucorum, Adelphocoris suturalis), This article analyzed of the growth and development parameters and the life table parameters of Apolygus lucorum and Adelphocoris suturalis feeding on different varieties of cotton systemative; We used DC-EPG to studied electrical penetration graph of Apolygus lucorum and Adelphocoris suturalis; and measured the activity change of three kind of detoxifying enzymes and AChE of Apolygus lucorum feeding on different varieties of cotton, the results are as fellows:1. Analysis of the growth and development parameters and the life table parameters of Apolygus lucorum and Adelphocoris suturalis feeding on transgenic cotton varieties and their corresponding maternal cotton varietiesThe growth and development parameters and the life table parameters of Apolygus lucorum and Adelphocoris suturalis feeding on transgenic cotton and their corresponding maternal cotton were analyzed at the photoperiod of 16 hrs light:8 hrs dark(26℃±1.0℃ and 75%±5% RH).The results showed that transgenic cotton varieties had a significant effect to Adelphocoris suturalis, Significant differences all existed on eggs duration, hatching rate of eggs, nymph duration, survival rate of nymph, oviposition, fecundity, preoviposition and weight; The length and width of Adelphocoris suturalis also had significant difference; The mean length of a generation feeding on transgenic cotton were 31.7232 d,32.0157 d,30.5141 d, while they were 24.5404 d, 26.5533 d,28.0263d feeding on maternal cotton; The survival rate of nymph feeding on transgenic cotton were 41.67%,40.00%,31.88%, while they were 50.00%,47.50%,45.00% feeding on maternal cotton; the intrinsic rate of natural increase on transgenic cotton were 0.0545, 0.0508,0.0549, while they were 0.0959,0.0865,0.0788 on maternal cotton.The growth and development parameters and the life table parameters on transgenic cotton and their corresponding maternal cotton showed that the transgenic cotton had a significant inhibitory action to Apolygus lucorum. The net reproductive rate of Apolygus lucorum on maternal cotton were 1.92,1.97,1.74 times as high as on transgenic cotton; The mean length of a generation feeding on transgenic cotton reached 32.3 d, while the lowest was only 22.3 d on their maternal cotton, the differ value reached up to 10d; the doubling time on transgenic cotton were 2.01,1.71,1.48 times as long as on maternal cotton; however, the index of population trend on maternal cotton were 2.70,2.56,2.65 times as large as on transgenic cotton; the intrinsic rate of natural increase on maternal cotton were 2.01,1.71,1.49 times as high as on transgenic cotton.2. Study of electrical penetration graphs of Apolygus lucorum and Adelphocoris suturalis on different cotton varietiesThe probing behaviors of Apolygus lucorum and Adelphocoris suturalis on transgenic cotton varieties and their corresponding maternal cotton varieties were recorded with DC-EPG. Comparison of the EPG parameters of Apolygus lucorum feeding on transgenic cotton varieties and their corresponding maternal cotton varieties:(1) The probe number was significantly different on transgenic cotton and maternal cotton, (2) The A duration within six hours and the A duration per probe on transgenic cotton and maternal cotton were significantly different; The M duration within six hours and the M duration per probe on transgenic cotton and maternal cotton also had a significant difference. (3) the B duration in 6hs and in per a probe on transgenic cotton were significantly less than on maternal cotton, the transgenic cotton took a definitely inhibitory action to Apolygus lucorum; the NP duration of Apolygus lucorum on transgenic cotton were extremely longer than the NP duration on their maternal cotton.Comparison of the EPG parameters of Adelphocoris suturalis feeding on transgenic cotton varieties and their corresponding maternal cotton varieties:(1) feeding on transgenic cotton, the probe duration, Ⅲduration in 6hs and in per a probe were significantly less than those on their corresponding maternal cotton. (2) Feeding on transgenic cotton, Ⅱ duration in 6hs were more than on maternal cotton; Ⅱ duration and the rate of Ⅱ in per a probe were more than those on their maternal cotton. (3) The probe number of Adelphocoris suturalis feeding on transgenic cotton was significantly more than it on maternal cotton in 6 hs, and the arisen rate of Ⅲ in a probe on transgenic cotton were less than it on their maternal cotton.3. Activity test of several kind of detoxifying enzyme and AChE of Apolygus lucorum on different cotton varietiesThe ACP and AKP activity were all significantly inhibited behind feeding on transgenic cotton varieties, along with time, the inhibitory action would gradually strengthen.; The effect of three kind of transgenic cotton to GST activity was different. (3) The model of action of transgenic cotton to AChE activity were extremely simple, it presented inhibitory action on A and C as a whole, but it could not take certain effect to AChE. |
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