Effects Of Different Casing Soil On Cultivation Of Agaricus Bisporus And Study Of Microbes In Casing Soil

Agaricus biaporus is the most widely cultivated of edible fungi in the world. Casing soil layer is very important for the cultivation of Agaricus biaporus. This study was focused on three aspects:a) the effects of different casing soils on the growth,yield and quality of Agaricus bisporus; b) the change of microbial population and DGGE map of casing soil in different stage, to analyzing the cultured bacterial population; c) screening bacterias which promote pining of Agaricus bisporus.The major results were summarized as follows:1 The properties of different casing soilsThe holding rate and porosity became larger with the increasing of content; The casing soil bulk density decreased and the electrical conductivity increased with increasing content of peat.2 Effects of different casing soils on the growth, yield and quality of Agaricus bisporusThe casing soil contains more peat is better to the growth of hypha, meanwhile, the yield of mushroom were relativerly higher. As a result, the content of peat influenced the yield of Agaricus bisporus significantly.In conclusion, soil with 50% peat was highly recommended to pratical agricultural application taken the cost, yield, quality into a commercial account.3 Microbial community structure of casing soil during Agaricus bisporus growthThere were the largest number of bacteria in microbial; The number of bacteria increased with the hypha growth, and reach peak during the primordium formation. The number of fungi and actinomycetes was less than the number of bacteria, changing slightly after casing.4 Microbial diversity of casing soil during Agaricus bisporus growthThe bands in the bacterial DGGE maps of the different casing soil in the distinct growing-phases differed in numbers and lighteness, which indicated that the diverseities of the bacterial communities in the different casing soil were different. With the groeth of Agaricus bisporus, the amount of bands and lighteness in the DGGE map was enhanced.