| The Amur tiger have genetic conservation value, belonging to the first level of Protection in our country. Due to overfishing, low reproduction rate, survival environment and food chain destroyed, used to be widely distributed in China’s northeast now have become a critically endangered species, as a result, research on its artificial breeding is very important. Siberian tigers’living environment dimension is higher, cold climate, Siberian tigers can withstand the low temperature, but high temperature tolerance is limited, especially climate humid area south of the Yangtze, the Amur tiger in the summer heat stress environment, activity decline, feed intake, reduce symptoms such as Poor, body condition. To alleviate the Amur tiger high temperature stress is one of the important measures to maintain summer the Amur tiger normal growth Performance.VE Play an important role for Poultry, livestock heat stress aspects, but less research on the Amur tiger on heat stress relief has been made, the study on Nanjing region climate investigate VE the Amur tiger behavior under high temperature and thermal stress, free radical metabolism, blood biochemical indexes and the change of blood cortical levels, in the hope of the heat stress effect mechanism of Siberian tigers to clarify and thermal stress caused by disease or Physical disorders Prevention and cure to give theoretical support, the results are as follows:(1) Focus observation method was used and the interval was five minutes, observe 4 Amur tiger in 5,7,8 at 8:00am, then during the day and hold three time breathing rate of early 6:30-9:30 and 11:30-14:00 at noon, two Period behavior and breathing after adding 700 IU of vitamin E and behavioral changes. It is found that high temperature environment compared with normal temperature environment, the Manchurian tiger breathing rate increased significantly (P< 0.05), and added in the diet of vitamin E in improving respiratory frequency have significant relief (P< 0.05); High temperature stress increase the Amur tiger morning walk, swimming behavior (P< 0.05), in the middle of the stand, running behavior reduced (P< 0.05), stomach, less rest half supine Position (P< 0.05), water increased (P< 0.05), walking, swimming behavior also increased, but the difference was not significant; Vitamin E is added in the diet after two weeks, walking behavior was down in the morning, at noon drinking behavior, dog sit behavior reduced (P< 0.05), running behavior has a tendency to increase, but the difference was not significant, lying Posture change, interest in the morning side decreased, Prone to increase (P< 0.05), till noon, Prone to reduce (P< 0.05), is given Priority to with side. This shows that high temperature stress cause adverse effect to the Amur tiger body, heat stress make the Amur tiger breathing rate increases, distribution of behavior change, and add vitamin E for relief of high temperature stress has a certain effect.(2) Compared to high temperature stress under the condition of different group of Amur tiger changes of free radical metabolism in the blood, the results showed that adding VE gsh-Px in the diet group, respectively, the high temperature control, high temperature heat stress group increased 1.48,3.51 times (P< 0.05), MDA content decreased significantly (P< 0.05), while with the normal control group no significant difference (P> 0.05); Add VE diet group and the SOD content in high temperature control group difference was not significant (P> 0.05), more than 23.92% higher than that of high temperature and heat stress group (P< 0.05); High temperature thermal stress in the blood-T AOC content is 9.57% lower than normal control group(P< 0.05), add VE diet groups with significant difference was found in normal control group (P< 0.05),22.60% higher than in the high temperature heat stress group (P< 0.05). That under the condition of high temperature stress in the Amur tiger, VE is added to the diet can significantly reduce the content of free radicals in the blood; alleviate the negative impact of the high temperature stress on the body.(3) Compared to 5 (control group) at room temperature, and high temperature (control group),8 group added (VE) in different experimental 15:00 PM during the east-northern tiger cortical concentration, blood biochemical indexes and the result shows:the heat stress group, the total number of white blood cells is significantly lower than normal control group (P< 0.05), after adding VE in the diet, total number of white blood cells increased, significantly higher than that of heat stress group (P< 0.05), but still below normal control group (P< 0.05), while red blood cell count is higher than normal control group (P< 0.05), and the average red blood cell volume was significantly lower than normal control group and high temperature stress group (P< 0.05); High temperature stress group of red blood cell hemoglobin concentration significantly lower than the rest of the treatment group (P< 0.05), Platelet count was significantly lower than the normal control group (P< 0.05). This shows that the thermal stress change the east-northern tiger blood biochemical indexes, increased cortical lees in the blood, and add a VE to heat stress relief. |
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