Analyze Of Fruit Main Characters And Survey Of Sequence-related Amplified Polymorphism(SRAP)marker Of 22 Plum Materials(Strains)

22 plum cultivars(strains) from 5 districts in Dazhou city Sichuan province including Tongchuan district, Wanyuan city, Xuanhan country, Dazhu country, Kai country were used as test materials in this experiment. Fruits main characters and sequence-related amplified polymorphism (SRAP)marker were analyzed in this study. The main results of this study are as follows:1.Fruits main characters such as exterior quality,interior quality etc. of 22 plum cultivars (strains) were unstable and inconsistent. Bashan Cuili showed the big average fruit weight, high sugar acid ratio and high soluble solids. The fruit qualities of Bashan Cuili were better than any other 21 plum cultivars (strains). Very late varieties (strains) from Wanyuan city were significantly lower than in other regions varieties (strains), total acid content was significantly higher than other regions. Early maturing varieties from Daxian (strains) and the rest did not show significant differences. And it did’t show significant differences between maturity and late-maturing varieties (strains). Differences in fruit traits, showing the performance of long circular (fruit shape index greater than 1.100), round (fruit shape index 1.000-1.100), flat round (fruit shape index of less than 1.000).2.Genomic DNA extraction methods of plum cultivars (strains) in Dazhou region were improved and SRAP-PCR system was optimized. CTAB method of Genomic DNA extraction method were improved. Shorten the bath time, the time was shorten from 0.5h to 10min,and adding chloroform/iso-amyl alcohol (24:1) times of extraction, the extraction times increased from 2 to 3 times. Using modified CTAB method of genome DNA electrophoresis, the electrophoresis in the genomic DNA of belt type was clear, high brightness, point sample hole brightness was not obvious, and the results showed that using the improved CTAB method could get high quality genomic DNA concentrations. The use of nucleic acid protein detected using genomic DNA extracted improved CTAB method, the results showed that D260nm/D280nm between 22 plum cultivars (strains) of genomic DNA arranged from 1.71 to 1.91.Detecting bands by Gel-Pro analyzer software, the nine orthogonal design combined most abundant bands. Analyzed the bands with orthogonal design of visual analysis method,the nine orthogonal design got the highest score. Comprehensive two methods to obtain the optimum reaction system SRAP marker was 1.5mmol/L dNTPs,3mmol/L Mg2+,1.25μmol/L primers,0.5ng/20 μL genomic DNA,0.4U/25 μL Taq DNA polymerase.3.Providing a theoretical basis for the identification of’Bashan Cuili’and protection and utilization of other crisp varieties in Dazhou, the Sequence related amplified polymorphism was used to analyze the genetic relationship of the Bashan Cuili and the excellent regional resources in Dazhou. twelve primer pairs which were high polymorphism and showed clear bands were selected while one hundred and eighty primer pairs of sequence related amplified polymorphism (SRAP) marker were used to analyze genetic diversity of 22 samples, generated 103 bands,64 bands (60.34%) of which were polymorphic ones. Each primer combination amplified loci was 5.42. A dendrogram was constructed based on SRAP data using UPGMA cluster method. The 22 samples of plum were classified into 3 major groups when the similarity coefficient was at 0.68.The clustering results and fruit shape was basically corresponded, and was related with resource distribution and mature phase.(When the similarity coefficient was at 0.72,Bashan Cuili was separated as a group, proved the genetic difference between Bashan Cuili and other 21 cultivars (strains).Dazhu Jinxin plum as the only red peel color cultivar, was not grouped separately, showed that there was not significant correlation between peel color and clustering result.