Studies On The Medicinal Plant Virus Diseases In Liaoning Province

In recent years, the planting areas of medicinal plants continue increasing. Virus diseases of medicinal plants are becoming more and more serious. Howerc, because of lacking systematic research on the medicinal plant virus diseases in the domestic, most virus types of medicinal. plants are unclear. This paper summarized most researches of medicinal plant virus and surveyed the incidences of nine kinds of medicinal plants in Liaoning Province and identified pathogens of the medicinal plant virus diseases systematically. Now the innovative research reported as follows:1. In the Acanthopanax sessiliflorus fields in Fengcheng Dandong, it was found that a large number of plants were infected with virus. The mainly symptoms were mosaic, yellow edge, shrinkage, leaves half green and half yellow and so on. The incidence of disease was above 60%. The results of mechanical inoculation showed that indicator plants weren’t infected. There were no results by serological detections with TMV, CMV, PVY, PVX and WMV five kinds of antiserums. Then,4 samples of Acanthopanax sessiliflorus were sent to Biomarker which is a sRNA deep sequenceing research company to do sRNA deep sequenceing detection. The result showed there might be Garlic virus (GV), Papaya ringspot virus(PRSV), Stealth virus(SV), Citrus exocorits viroid(CEVd) and other two kinds of virus in the Acanthopanax sessiliflorus samples.Then articles were consulted and specific primers were dcsigned. By RT-PCR detection, it was found that there was CEVd in Acanthopanaxe sessiliflorus. The nucleotide sequence analysis indicated that the identity of CEVd between Acanthopanaxe sessiliflorus and Mexic CEVd strain 7(HE818450) was 99.7%. This is a first report about Acanthopanaxe sessiliflorus virus disease.2. In partial of planting areas of medicinal plants in Liaoning Province, there were some medicinal plants, such as Schisandra chinensis, Agrimonia pilosa, Atracylode chinensis, and Physali alkekengi L which have mosaic, yellowing, shrivel, chlorosis, dwarf and other virus like disease symptoms. The mechanical inuclations results showed that Schisandra chinesesis, Agrimonia pilosa, Atracylode chinensis and Physali alkekengi L, were mosaic on the common tobacco NC89 and tomatoes. Nicotiana glutinosa showed necrosis. Gomphrena globosa and Camaranticolor amaranticolor showed necrosis and chlorisis and so on. Using the TMV, CMV, PVY, PVX and WMV five kinds of antiserums tested these plants. The results of ELISA were that Schisandra chinensis, Agrimonia pilosa, Atracylode chinensis, and Physali alkekengi L with TMV antiserum showed positive reaction. The serological detection of Atracylode chinensis with CMV antiserum showed positive reaction. Molecular biology tested these medicinal plants with specific primers of TMV P126, TMV CP(coating protein), CMV CP, PVY, PVX CP and WMV CP.The results showed that TMV were in Schisandra chinensis, Agrimonia pilosa, Atracylode chinensis and Physali alkekengi L. The analysis of nucleotide sequence showed that the identities of TMV CP among Schisandra chinensis, Physali alkekengi L and almond 1 (HE818450) from Shandong Province were the highest. The identity of TMV CP between Agrimonia pilosa and almond 2(HE818449) from Shandong Province was the highest. The identity of TMV CP between Atracylode chinensis and tobacco from Chengjiang (HE818410) was 100%. These are first reports in China. Howere, there wasn’t CMV in Atracylode chinensis by RT-PCR. According to the results of mechanical inuclation and serology, CMV was highly possible in Atracylode chinensis. Therefore, it needs to further research and analysis.3. Investigation in fields showed that Panax gensing, Glehnia littoralis, Artium lappa L and Pulsitilla chinensis have yellowing, vein necrosis, mosaic and other virus like disease symptoms. The sap mechanical inoculations showed that these medicinal plants samples can infect Gomphrena globosa and Camaranticolor amaranticolor with mosaic, chlorosis and leaf spot symptoms. Antiserums of the CMV、TMV、PVY、PVX and WMV were used for testing by the ELISA, which showed that Panax gensing and Glehnia littoralis samples appeared positive reaction with PVY antiserum, but negative reaction with other antiserums.While Artium lappa L and Pulsitilla chinensis samples expressed negative reactions with these 5 kinds of antiserums. In conclusion, Panax gensing and Glehnia littoralis might conclude PVY, but we need research carefully worked-out.