The Effect Of Compound Enzyme On The Digestibility Of Several Kinds Of Feed Ingredients Nutrients And Its Application In The Swine Feedstuff

This experiment analyzed the influence of compoundenzyme on digestibility of feed ingredients nutrientsand the influence on production performance of pig throughthe digestion test in vitro and animal feeding test. Results are as follows:1. In vitro digestion experiments. In order to clarify the effect ofcompound enzyme on the nutrient digestibility of corn, soybean meal, wheat bran, DDGS, cottonseed meal, rapeseed meal, we determinated digestibility of dry matter, energy, crude protein, crude fiber, neutral detergent fiber, acid detergent fiber, calcium and phosphorusby simulation tests in vitro including three-step tests such as gastric digestion,intestinal digestion and hindgut digestion. The test was divided into two groups,one group with the same 0.1% compoundEnzymewhile other group without.The test results showed that thecomposite enzyme could improve the digestibility of CP, P, Lys significantly (P＜0.05). The impact of differences in other indicators such as digestibility of dry matter were not significant (P>0.05), but there were tendencies to be improved. Composite enzyme could improve the digestibility of P in soybean meal electrode significantly (P＜0.01), and improvethe digestibility of GE, DM, CP, CF, NDF, ADF and Cyssignificantly (P<0.05). The impact of the differences on the part of the amino acid digestibility was not significant (P>0.05), but there is tendency to be improved. Compositeenzymeimprovedthe digestibility of wheat bran and Met DM significantly (P0.01), and improved the digestibility of GE, P, CF, NDF, ADF, CP, Thr and Try significantly (P0.05). The impact of differences in digestibility of lysine and cystine was not significant (P＞0.05), but there were tendencies to be improved. Enzymeimproved the digestibility of DDGS P and ADF significantly (P＜0.01), and improved the digestibility of GE, CF and NDF significantly (P＜0.05). The impact of differences in other indicators such as digestibility of dry matter was not significant (P＞0.05), there were tendencies to be improved. Enzymeimproved the digestibility of NDF and cottonseed meal ADF significantly (P＜0.01), and improved the digestibility of GE, CF, DM, CP, P, Met and Thrsignificantly (P＜0.05). The impacts of differences in Lys, Cys and Try digestibility were not significant (P＞0.05), but there were tendencies to be improved. Composite Enzymeimproved the digestibility of meal in P, NDF and ADF significantly (P＜0.01), and improved the digestibility of GE, DM, CP, CF and Lys significantly (P＜0.05). The impact of the partial amino acid digestibility difference was not significant (P＞0.05), but but there is tendency to be improved, enzyme also quite different for different kinds of diets with nutrient digestibility.2. The animal feeding experiments. Choosing 40 DLY growingpigs with the same weight about 25 Kg, and divided these pigs into two treatments in male and female halves principle. And every treatment were with 5 replicates of 4 pigs. The control group was fed with the basal diet, and the test group based on enzyme basis added 0.1mg/kg diet. Feeding experiments lasted 60 days, and the test started and ended of the fasting individual weighed. Feed consumption was used to calculate the average daily gain of pigs (ADG), feed conversion ratio (F/G), average daily feed intake amount (ADFI), diarrhea rate and blood biochemical parameters by repeating set of statistics during the test.The test results showed that the daily gain was increased significantly by 3.57% (P＜0.05), feed intake and feed conversion ratio decrease were not significant (P>0.05), while diarrhea rate decreased signify cantlyby 52.3%(P＜0.05); when the diet adding enzyme group was compared with control group. And compared with control group, The contents of serum total protein, albumin and globulin increased significantly (P＜0.05), by 8.25%,8.74% and 7.69% respectively. The contents of aspertate aminotransferase, and alkaline phosphatase were reduced significantly by 4.52%, and 11.85% respectively (P＜0.05), while the contents of glutamic-pyruvic trans aminaseand lactate dehydrogenase was reduced insignificantly (P＞0.05); The contents of blood glucose and serum cholesterol increased, insignificantly (P＞0.05). The content of serum urea nitrogen reduced, significantly by 8.02%(P＜0.05), compared with the control group, while which of serum triglyceride was reduced, insignificantly (P＞0.05).In summary, the special commodity-type enzyme selected by this study could significantly improve digestibility of several feed ingredients for most nutrients. The test feed could improve growth performance and blood biochemical parameters of growing pigs after adding enzyme.